In Vitro Induction of Tetraploidy in Vaccinium darrowi, V. elliottii, and V. darrowi X V. elliottii with Colchicine Treatment

in Journal of the American Society for Horticultural Science
Authors:
J. L. PerryFruit Crops Department, University of Florida, Gainesville, FL 32611

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P. M. LyreneFruit Crops Department, University of Florida, Gainesville, FL 32611

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Abstract

Three diploid taxons (Vaccinium darrowi Camp, V. elliottii Chapm., and interspecific V. darrowi x V. elliottii) were treated with various colchicine concentrations and treatment durations to determine the best method for inducing autopolyploidy in in vitro blueberry cultures. Shoot-tip cuttings were the best in vitro planting material for induction of shoots with increased diameter, an indicator of polyploidy. Tetraploids were produced at colchicine concentrations from 0% to 0.20%. The best treatment combinations were genotype-dependent.

Contributor Notes

Received for publication 14 Apr. 1983. University of Florida Journal Series No. 4677. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.

Graduate Research Assistant.

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