Hormonal Control of Strawberry Axillary Bud Development in Vitro1

in Journal of the American Society for Horticultural Science
Authors:
Kimani WaithakaDepartments of Horticulture and Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706

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Albert C. HildebrandtDepartments of Horticulture and Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706

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Malcolm N. DanaDepartments of Horticulture and Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706

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Abstract

In vitro cultures were used to study the development of axillary bud and stolon tip explants of cultivated strawberry (Fragaria × ananassa Duch.). Explants cultured on Murashige-Skoog basal media containing kinetin at 1, 5, or 10 mg/liter developed into leafy shoots. Low concentration of kinetin (1 mg/liter) promoted the development of both types of explants into single shoots while higher concentration (10 mg/liter) promoted production of multiple leafy shoots developing from axillary buds of the earlier formed leafy shoots. NAA at 1 mg/liter promoted callus growth from both types of explants. Axillary bud explants developed into stolons when cultured on media containing gibberellic acid (GA3) at 5, 10 or 20 mg/liter. Stolon apices developed into leafy shoots while the second axillary stolon buds of the tips were inhibited when the explants were cultured on GA3-containing media. Combinations of GA3 and kinetin induced the development of axillary bud explants into structures intermediate in form between those of stolons and leafy shoots. Stolon apices and stolon axillary buds at the stolon tips developed into leafy shoots and continuing stolons, respectively, when the explants were cultured on a kinetin-containing medium for one week, and then transferred onto a GA3-containing medium. Thus, the developmental pathway of axillary strawberry buds was shown to be responsive to a balance between GA and cytokinins following removal from apical dominance.

Contributor Notes

Received for publication January 22, 1979. Research supported by the Research Division, College of Agricultural and Life Sciences.

The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper must therefore be hereby marked advertisement solely to indicate this fact.

Research Assistant and Professors, respectively.

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