In Vitro Propagation of Japanese Plum (Prunus salicina Lindl. cv. Calita)1

in Journal of the American Society for Horticultural Science
Authors:
Pasquale RosatiCentro di Studio per la Tecnica Frutticola del C.N.R., Bologna 40126, Italy

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Grazia MarinoCentro di Studio per la Tecnica Frutticola del C.N.R., Bologna 40126, Italy

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Chiara SwierczewskiCentro di Studio per la Tecnica Frutticola del C.N.R., Bologna 40126, Italy

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Abstract

Tissue cultures of ‘Calita’ Japanese plum started from 5 to 7 mm long shoot tips proliferated at a rate of 10:1 to 20:1 per month when grown on a modified Murashige and Skoog medium with 3% sucrose, 0.75% agar and (in mg/liter) 0.4 thiamine HCl, 100 myo-inositol, 1.0 6-benzylamino purine (BA), 0.1 gibberellic acid (GA3) and 0.1 indolebutyric acid (IBA). Best rooting results were obtained with 2 or 4 mg/liter indolebutyric acid (IBA) at 21°C; raising the temperature to 26° or 30° slowed and decreased rooting unless 0.1 mg/liter GA3 was included in the medium. Activated charcoal in the medium drastically reduced rooting. Rooted cuttings were easily established in soil.

Contributor Notes

Received for publication June 9, 1979. Work supported by the C.N.R. Research Project “Biology of Reproduction,” Subproject 7 “Micropropagation.” Scientific Paper No. 123.

The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper must therefore be hereby marked advertisement solely to indicate this fact.

Research Scientist, C.N.R.

Graduate student supported by a C.N.R. grant.

Graduate student supported by a grant from Banca Operaia, Bologna.

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