Formation of Adventitious Embryos in Callus Cultures of ‘Seyval’, a French Hybrid Grape1

in Journal of the American Society for Horticultural Science
Authors:
William R. KrulAgricultural Research Service, U. S. Department of Agriculture, Beltsville, MD 20705

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Joseph F. WorleyAgricultural Research Service, U. S. Department of Agriculture, Beltsville, MD 20705

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Abstract

Callus tissue of ‘Seyval’ (Seyve-Villard 5-276) (Vitis sp.), a French hybrid grape, formed adventitious embryos when transferred from a medium with 2,4-dichlorophenoxyacetic acid (2,4-D) to a medium containing 1-napthaleneacetic acid (NAA). Embryos began to turn green and develop into apparently normal vines when placed on a medium free of hormones and vitamins in the light. Histological evidence indicated that plants derived from callus originated from embryo-like structures and not from plantlets or excised buds. Secondary embryoids formed on primary embryoids, and tertiary embryoids occasionally formed on secondary embryoids. More than 50 vines and several hundred adventitious embryoids were obtained from approximately one cubic centimeter of callus.

Contributor Notes

Received for publication January 11, 1977.

Cell Culture and Nitrogen Fixation Laboratory, Plant Physiology Institute.

Plant Hormone and Growth Regulators Laboratory, Plant Physiology Institute. We thank Susan Alsop for her excellent technical assistance and for 100% success in obtaining vigorous grapevines from adventive embryoids.

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