Recurrent Full-sib Family Selection for Husk Extension in Sweet Corn

in Journal of the American Society for Horticultural Science

Corn earworm (Helicoverpa zea) is a costly and destructive pest in sweet corn (Zea mays) production. A recurrent selection experiment was conducted to assess the feasibility of selecting a sweet corn population for longer husks without shortening the ears and to determine whether direct selection for longer husks confers improved resistance to the corn earworm. The initial population was derived from crosses between long- and tight-husked inbred lines (A684su, A685su, and A686su) originally selected for resistance to the european corn borer (Ostrinia nubilalis) and a high-quality commercial hybrid parent (‘Super Sweet Jubilee Plus’). Three cycles of recurrent full-sib family selection were conducted from 2014 to 2016, with a selection intensity of ≈10% per cycle. In 2017, the cycles of selection were evaluated and compared with common commercial cultivars for husk extension, ear length, rate of corn earworm infestation, and extent of corn earworm damage. Ears were artificially infested with corn earworm eggs suspended in an agar solution. The population was successfully selected for longer husk extension without shortening the ears, but the increase in husk extension did not correspond to an increase in corn earworm resistance. Further evaluation is needed to determine whether long husk extension is ineffective as a source of corn earworm resistance, whether it is more effective in combination with other resistance traits, whether greater differences in husk extension are needed to confer biologically significant differences in corn earworm resistance, or whether improved inoculation methods could improve differentiation between genotypes.

Contributor Notes

Current address: Department of Crop & Soil Sciences, NC State University, Campus Box 7620, Raleigh, NC 27695.

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Article References

BarberG.W.1936The cannibalistic habits of the corn ear worm. U.S. Dept. Agr. Tech. Bul. 499

BarberG.W.1941Observations on the egg and newly hatched larva of the corn ear worm on corn silkJ. Econ. Entomol.343451456

BarberG.W.1944Husk development of sweet corn as affected by moisture supply, an important factor in corn earworm controlJ. Agr. Res.6827377

BartonK.2018MuMIn: Multi-model inference. R package version 1.40.4. 22 Aug. 2018. <>.

BatesD.MaechlerM.BolkerB.WalkerS.2015Fitting linear mixed-effects models using lme4J. Stat. Softw.671148

ButronA.WidstromN.W.SnookM.E.WisemanB.R.2002Recurrent selection for corn earworm (Lepidoptera: Noctuidae) resistance in three closely related corn southern syntheticsJ. Econ. Entomol.952458462

CollinsG.N.KemptonJ.H.1917Breeding sweet corn resistant to the corn earwormJ. Agr. Res.112549572

CookR.CarterA.WestgateP.HazzardR.2003Direct silk applications of corn oil and Bacillus thuringiensis as a barrier to corn earworm larvae in sweet cornHortTechnology13509514

CookR.CarterA.WestgateP.HazzardR.2004Optimum timing of an application of corn oil and Bacillus thuringiensis to control lepidopteran pests in sweet cornHortTechnology14307314

DavisD.W.AndowD.A.HutchisonW.D.1993Registration of three european corn borer resistant sweet corn germplasm lines: A684su, A685su, and A686suCrop Sci.33614221423

Del ValleC.G.MillerJ.C.1963Influence of husk length and tightness against corn earworm damage in sweet corn hybridsProc. Amer. Soc. Hort. Sci.83531535

DickeF.F.BarberG.W.1944Husk characters of field corn in relation to feeding by birds on earwormsJ. Econ. Entomol.371119120

DouglasW.A.1947The effect of husk extension and tightness on earworm damage on cornJ. Econ. Entomol.405661664

HardwickD.F.1965The corn earworm complexMem. Entomol. Soc. Can.97S405247

HothornT.BretzF.WestfallP.2008Simultaneous inference in general parametric modelsBiometrical J.503346363

HuangF.2015Resistance management for Bt maize and above-ground lepidopteran targets in the USA: From single-gene to pyramided traits p. 173–185. In: M. Soberón (ed.). Bt resistance: Characterization and strategies for GM crops producing Bacillus thuringiensis toxins. CABI Biotechnol. Ser. 4. CAB Intl. Boston MA

KennedyG.G.StorerN.P.2000Life systems of polyphagous arthropod pests in temporally unstable cropping systemsAnnu. Rev. Entomol.451467493

KuznetsovaA.BrockhoffP.B.ChristensenR.H.B.2017lmerTest package: Tests in linear mixed effects modelsJ. Stat. Softw.8213126

KyleC.H.1918Shuck protection for ear corn. U.S. Dept. Agr. Tech. Bul. 708

LenthR.V.2016Least-squares means: The R package lsmeansJ. Stat. Softw.691133

MihmJ.A.1982Techniques for efficient mass rearing and infestation in screening for host plant resistance to corn earworm Heliothis zea. Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT) El Batan Mexico

NeunzigH.H.1963Wild host plants of the corn earworm and the tobacco budworm in eastern North CarolinaJ. Econ. Entomol.562135139

NiX.XuW.KrakowskyM.D.BuntinG.D.BrownS.L.LeeR.D.CoyA.E.2007Field screening of experimental corn hybrids and inbred lines for multiple ear-feeding insect resistanceJ. Econ. Entomol.100517041713

NiX.KrakowskyM.D.BuntinG.D.RectorB.G.GuoB.SnookM.E.2008Identification of multiple ear-colonizing insect and disease resistance in CIMMYT maize inbred lines with varying levels of silk maysinJ. Econ. Entomol.101414551465

NiX.SparksA.N.RileyD.G.LiX.2011Impact of applying edible oils to silk channels on ear pests of sweet cornJ. Econ. Entomol.1043956964

NiX.XuW.BlancoM.H.WilsonJ.P.2012Evaluation of corn germplasm lines for multiple ear-colonizing insect and disease resistanceJ. Econ. Entomol.105414571464

OlmsteadD.L.NaultB.A.SheltonA.M.2016Biology, ecology, and evolving management of Helicoverpa zea (Lepidoptera: Noctuidae) in sweet corn in the United StatesJ. Econ. Entomol.109416671676

PainterR.H.BrunsonA.M.1940Differential injury within varieties, inbred lines, and hybrids of field corn caused by the corn earworm, Heliothis armigera (Hbn.)J. Agr. Res.61281100

PhillipsW.J.KingK.M.1923The corn earworm: Its ravages on field corn and suggestions for control. U.S. Dept. Agr. Farmers’ Bul. 1310. 10 Sept. 2018. <>.

PimentelD.FriedmanJ.KahnD.1997Reducing insecticide fungicide and herbicide use on vegetables and reducing herbicide use on fruit crops p. 379–397. In: D. Pimentel (ed.). Techniques for reducing pesticide use: Economic and environmental benefits. Wiley New York NY

ReisigD.D.Reay-JonesF.P.F.2015Inhibition of Helicoverpa zea (Lepidoptera: Noctuidae) growth by transgenic corn expressing Bt toxins and development of resistance to Cry1AbEnviron. Entomol.44412751285

SnyderR.J.1967The relationship of silk balling, husk length, husk tightness and blank tip to earworm and sap beetle resistance in maizeProc. Amer. Soc. Hort. Sci.91454461

SudbrinkD.L.GrantJ.F.1995Wild host plants of Helicoverpa zea and Heliothis virescens (Lepidoptera: Noctuidae) in eastern TennesseeEnviron. Entomol.24510801085

TabashnikB.E.CarriereY.2015Successes and failures of transgenic Bt crops: Global patterns of field-evolved resistance p. 1–14. In: M. Soberón. Bt resistance: Characterization and strategies for GM Crops producing Bacillus thuringiensis toxins. CABI Biotechnol. Ser. 4. CAB Intl. Boston MA

TabashnikB.E.Van RensburgJ.B.J.CarriereY.2009Field-evolved resistance to Bt crops: Definition, theory, and dataJ. Econ. Entomol.102620112025

WickhamH.2011The split-apply-combine strategy for data analysisJ. Stat. Softw.401129

WidstromN.W.1967An evaluation of methods for measuring corn earworm injuryJ. Econ. Entomol.603791794

WidstromN.W.BurtonR.L.1970Artificial infestation of corn with suspensions of corn earworm eggsJ. Econ. Entomol.632443446

WidstromN.W.McMillianW.W.WisemanB.R.1970Resistance in corn to the corn earworm and the fall armyworm. IV. Earworm injury to corn inbreds related to climatic conditions and plant characteristicsJ. Econ. Entomol.633803808

WisemanB.R.McMillianW.W.WidstromN.W.1970Husk and kernel resistance among maize hybrids to an insect complexJ. Econ. Entomol.63412601262

WisemanB.R.WidstromN.W.McMillianW.W.1974Methods of application and numbers of eggs of the corn earworm required to infest ears of corn artificiallyJ. Econ. Entomol.6717476

YadavR.P.1980Response of sweet corn hybrids to corn earworm, Heliothis zea (Boddie) damageSouthern Univ. Res. Bul.226775

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