Comparison of Defensive Enzyme Activities in the Leaves of Seven Oriental Lily Hybrids after Inoculation with Botrytis elliptica

in Journal of the American Society for Horticultural Science

Plant resistance characteristics are closely related to changes in the activities of self-defense enzymes after infection. Despite significant differences in the resistance of different lily (Lilium sp.) cultivars to leaf blight (Botrytis elliptica), few studies of their resistance physiology exist. This study explored changes in the resistance-related enzyme activity of several lily cultivars after leaf blight inoculation. Seven oriental lily cultivars (Lilium hybrids) with obvious differences in resistance were used as experimental materials. After inoculation with B. elliptica, the activities of four defense enzymes, superoxide dismutase (SOD), catalase (CAT), phenylalanine ammonia-lyase (PAL), and peroxidase (POD), were determined according to the light absorption values at different wavelengths after their reactions. The results showed that the activities of SOD and CAT differed between the highly resistant and highly susceptible hybrids. Before inoculation, SOD activity was relatively low in all cultivars. However, after inoculation, the SOD activity increased sharply in the resistant cultivars. In the moderately resistant cultivars, the SOD activity did not change drastically. In the susceptible cultivars, the SOD activity initially showed slight increases or decreases and then increased. CAT activity showed reactions similar to those of SOD. Some changes in PAL and POD activity occurred after inoculation, but no significant correlations were present between these trends and resistance characteristics. In addition, no significant changes in enzyme activities were found in the control plants of the seven cultivars during the testing period. Overall, the resistance of Lilium oriental hybrids to B. elliptica is related to SOD and CAT activity but does not show much of a relationship with PAL and POD activity. Studying the physiological metabolic pathways of SOD and CAT appears to be an important direction in research to elucidate resistance to B. elliptica in Lilium oriental hybrids.

Contributor Notes

This study was supported by Jiangsu University Brand Specialization Project Support Project (PPZY2015A063) and A Project Funded by the Priority Academic Program Development of JiangSu Higher Education Institution (PAPD), China.

The funders had no roles in the design, analysis, writing, or submission of this work.

Corresponding author. E-mail: hufengrcn@163.com

Article Sections

Article Figures

  • View in gallery

    Trends of changes in superoxide dismutase (SOD) activity in seven Lilium oriental cultivars after inoculation with Botrytis elliptica. (A) Changes in the uninoculated control group (CK) from day 1 to day 7. (B) Comparisons between the inoculated and CK plants of each cultivar.

  • View in gallery

    Trends of changes in peroxidase (POD) activity in seven Lilium oriental cultivars after inoculation with Botrytis elliptica. (A) Changes in the uninoculated control group (CK) from day 1 to day 7. (B) Comparisons between the inoculated and CK plants of each cultivar.

  • View in gallery

    Trends of changes in catalase (CAT) activity in seven Lilium oriental cultivars after inoculation with Botrytis elliptica. (A) Changes in the uninoculated control group (CK) from day 1 to day 7. (B) Comparisons between the inoculated and CK plants of each cultivar.

  • View in gallery

    Trends of changes in phenylalanine ammonia-lyase (PAL) activity in seven Lilium oriental cultivars after inoculation with Botrytis elliptica. (A) Changes in the uninoculated control group (CK) from day 1 to day 7. (B) Comparisons between the inoculated and CK plants of each cultivar.

Article References

ChangS.W.KimS.K.HwangB.K.2001Gray mold of day lily (Hemerocallis fulva L.) caused by Botrytis elliptica in KoreaPlant Pathol. J.17305307

ChenG.YanC.X.LiC.J.ZhangY.T.ShangS.H.2009Changes of peanut peroxidase and catalase activity before and after aflatoxin infectionShandong Agr. Sci.20091214(in Chinese with English abstract)

ChenJ.F.WangG.N.ChengS.M.2008Progress about catalase function in plant stress reactionsXibei Zhiwu Xuebao2008188193(in Chinese with English abstract)

ChoodamaniM.S.HariprasadP.SateeshM.K.UmeshaS.2009Involvement of catalase in bacterial blight disease development of rice caused by Xanthomonas oryzae pv. OryzaIntl. J. Pest Mgt.55121127

ChoudharyD.K.VarmaA.2016Microbial-mediated induced systemic resistance in plants. Springer Singapore

DatJ.F.PellinenR.BeeckmanT.Van De CotteB.LangebartelsC.KangasjärviJ.InzéD.Van BreusegemF.2003Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobaccoPlant J.33621632

de ArmasR.SantiagoR.LegazM.E.VicenteC.2007Levels of phenolic compounds and enzyme activity can be used to screen for resistance of sugarcane to smut (Ustilago scitaminea)Australas. Plant Pathol.363238

Ehsani-MoghaddamB.CharlesM.T.CarisseO.KhanizadehS.2006Superoxide dismutase responses of strawberry cultivars to infection by Mycosphaerella fragariaeJ. Plant Physiol.163147153

EladY.WilliamsonB.TudzynskiP.DelenN.EladY.WilliamsonB.2007Botrytis: Biology pathology and control. Springer Dordrecht The Netherlands

Espinosa-LealC.A.Puente-GarzaC.A.García-LaraS.2018In vitro plant tissue culture: Means for production of biological active compoundsPlanta2018118

FarahaniA.S.TaghaviS.M.2018Rutin promoted resistance of tomato against Xanthomonas perforansEur. J. Plant Pathol.151527531

GuanL.LuoF.GuoR.HuF.2012Effect of pH on the activity of the crude extract enzyme from lily leavesJiangsu Agr. Sci.40147149(in Chinese)

HameedA.IqbalN.2014Chemo-priming with mannose, mannitol and H2O2 mitigate drought stress in wheatCereal Res. Commun.42450462

HemmM.R.RiderS.D.OgasJ.MurryD.J.ChappleC.2004Light induces phenylpropanoid metabolism in Arabidopsis rootsPlant J.38765778

HuF.LiuG.HuY.GuoR.ZhuL.LuoF.WangF.2017Authenticity identification and leaf blight resistance evaluation of the F1 hybrids from two Lilium, cultivars ‘Sorbonne’ and ‘Francia’Physiol. Mol. Plant Pathol.100194200

HuangJ.2001Plant pathogenesis and resistance. Springer Dordrecht The Netherlands

IghodaroO.M.AkinloyeO.A.2017First line defence antioxidants-superoxide dismutase (SOD) catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J. Med.doi: 10.1016/j.ajme.2017.09.001

JayarajJ.BhuvaneswariR.RabindranR.MuthukrishnanS.VelazhahanR.2010Oxalic acid-induced resistance to Rhizoctonia solani in rice is associated with induction of phenolics, peroxidase and pathogenesis-related proteinsJ. Plant Interact.5147157

JonesJ.1996Plant pathology. Paranoid plants have their genes examinedCurr. Biol.4749751

JonesJ.D.G.DanglJ.L.2006The plant immune systemNature444323329

KawaokaA.MatsunagaE.EndoS.KondoS.YoshidaK.ShinmyoA.EbinumaH.2003Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspenPlant Physiol.13211771185

LeeS.C.HwangB.K.2005Induction of some defense-related genes and oxidative burst is required for the establishment of systemic acquired resistance in Capsicum annuumPlanta221790800

LiC.1994Causes and countermeasures of summer death of edible lily in GansuPlant Protection Technol. Ext.19941314(in Chinese with English abstract)

MaX.ZhuD.2003Functional roles of the plant superoxide dismutaseHereditas25225231(in Chinese)

McRaeE.A.1998Lilies: A guide for growers and collectors. Timber Press Portland OR

ModafarC.BoustaniE.RahiouiB.MezianeA.Alaoui-TalibiZ.2006Suppression of phenylalanine ammonia-lyase activity elicited in date palm by Fusarium oxysporum f. sp. albedinis hyphal wall elicitorBiol. Plant.50697700

MollaeiS.FarahmandH.TavassolianI.2018The effects of 24-epibrassinolide corm priming and foliar spray on morphological, biochemical, and postharvest traits of sword lilyHort. Environ. Biotechnol.5919

MurL.A.J.KentonP.AtzornR.MierschO.WasternackC.2006The outcomes of concentration-specific interactions between salicylate and jasmonate signaling include synergy, antagonism, and oxidative stress leading to cell deathPlant Physiol.140249262

PandeyV.TewariA.K.SaxenaD.2017Activities of defensive antioxidant enzymes and biochemical compounds induced by bioagents in Indian mustard against alternaria blightProc. Natl. Acad. Sci. India2110

PatelS.J.SubramanianR.B.JhaY.S.2011Biochemical and molecular studies of early blight disease in tomatoPhytoparasitica39269283

SantiagoR.ArmasR.D.BlanchM.VicenteC.LegazM.E.2010In vitro effects of caffeic acid upon growth of the fungi Sporisorium scitamineumJ. Plant Interact.5233240

SiddiqueZ.AkhtarK.P.HameedA.SarwarN.HaqI.U.KhanS.A.2014Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virusJ. Plant Interact.9702711

SongX.Y.2016Physiological responses of Asian lilies under Botrytis cinerea stress and cloning of the regulatory gene MAPK. Diss. Shenyang Agr. Univ. Shenyang China (in Chinese with English abstract)

SrivastavaS.SinghA.2010Changes in catalase activity and total protein content in urdbean [Vigna mungo (L.) Hepper] plants as a result of ULCV infectionIndian J. Soc. Res.16769

SunH.LiT.LiY.2004Changes of phenols content and activity of enzymes related to phenols in lily bulbs stored at different cold temperatures for breaking dormancyZhongguo Nong Ye Ke Xue3717771782(in Chinese with English abstract)

TahsiliJ.SharifiM.SafaieN.Esmaeilzadeh-BahabadiS.BehmaneshM.2014Induction of lignans and phenolic compounds in cell culture of Linum album by culture filtrate of Fusarium graminearumJ. Plant Interact.9412417

TangX.XiaoA.YouC.ZengY.PengG.LiuW.ZhangD.1998A study on the biological properties of Botrytis elliptica causing lily gray mouldActa Agriculturae Universitis Jiangxiensis20485489(in Chinese with English abstract)

Van BaarlenP.WolteringE.J.StaatsM.van KanJ.A.2007Histochemical and genetic analysis of host and non-host interactions of Arabidopsis with three Botrytis species: An important role for cell death controlMol. Plant Pathol.84154

VanithaS.C.UmeshaS.2008Variations in defense related enzyme activities in tomato during the infection with bacterial wilt pathogenJ. Plant Interact.3245253

VanithaS.C.NiranjanaS.R.UmeshaS.2009Role of phenylalanine ammonia lyase and polyphenol oxidase in host resistance to bacterial wilt of tomatoJ. Phytopathol.157552557

WangS.LiY.LiC.1990A study on lily blightGansu Agr. Sci. Technol.19903032(in Chinese)

WeibergA.WangM.LinF.ZhaoH.ZhangZ.KaloshianI.HuangH.D.JinH.L.2013Fungal small RNAs suppress plant immunity by hijacking host RNA interference pathwaysScience342118123

YuH.ZhuH.LiuL.TangX.WeiL.2012PAL and POD activities of soybean hypocotyls infected by PythiumDadou Kexue31495497(in Chinese with English abstract)

ZhangY.GaoJ.ZhangQ.ZhangA.WangH.Sun.J.2016Research progress on plant superoxide dismutaseAnim. Husb. Feed Sci.372831(in Chinese with English abstract)

ZhaoC.WangA.ShiY.WangL.LiuW.WangZ.LuG.2008Identification of defense-related genes in rice responding to challenge by Rhizoctonia solaniTheor. Appl. Genet.116501516

ZhouB.2012Correlation between resistance of eggplant and defense-related enzymes and biochemical substances of leavesAfr. J. Biotechnol.111389613902

ZhuL.HuF.LuoF.2010Identification of disease resistance of Lilium species to gray mouldPlant Protection36148151(in Chinese with English abstract)

Article Information

Google Scholar

Related Content

Article Metrics

All Time Past Year Past 30 Days
Abstract Views 80 80 50
Full Text Views 32 32 12
PDF Downloads 18 18 3