Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

in Journal of the American Society for Horticultural Science

Antioxidants, antioxidant capacity, and the expression of isoprenoid metabolism–related genes and two pigmentation-related transcription factors were studied in four native and four hybrid tomato (Solanum lycopersicum) genotypes with different-colored fruit. Red fruit genotypes were associated with greater lycopene, β-carotene, lipophilic antioxidant capacity, and greater chromoplast-specific lycopene β-cyclase (CYC-B) transcript levels. Orange fruit genotypes had greater concentrations of tocopherols and greater transcript levels of homogentisate phytyl transferase (VTE-2), 1-deoxy-D-xylulose phosphate synthase (DXS), and 4-hydroxyphenylpyruvate dioxygenase (HPPD). The yellow fruit genotype was greater in total polyphenol and hydrophilic antioxidant capacity with greater expression of geranylgeranyl reductase (GGDR), phytol kinase (VTE-5), phytoene synthase (PSY) 2, lycopene β-cyclase (LCY-B), SlNAC1, and SINAC4. Greater levels of individual antioxidants were associated with specific coloration of tomato fruit. Moreover, the negative correlations between the expression of PSY1 and VTE-5, and between lycopene and chlorophyll, suggest a balance between carotenoids, tocopherols, and chlorophylls. The results of this study support either the direct commercialization of tomatoes with different color fruit or use of their genotypes in breeding programs to increase antioxidant levels among existing cultivars.

Contributor Notes

We thank CONACYT (Consejo Nacional de Ciencia y Tecnología; scholarship 265752) and UAM (Universidad Autónoma Metropolitana) for its financial support.

Corresponding author. E-mail: ljpf@xanum.uam.mx or laujperez@yahoo.com.mx.

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    Studied tomato genotypes and germplasm accession codes. Native genotypes (± se): red [RN (accession code 15014, hue 44.71 ± 0.94)], orange [ON (accession code 15006-3A, hue 70.3 ± 2.2)], yellow [YN (accession code 15011, hue 81.9 ± 2.3)], purple [PN (accession code 15440, hue 56.4 ± 1.2)]. Hybrid experimental lines: light red [LRH (accession code H1329, hue 45.2 ± 4.6)], light orange [LOH (accession code H1326, hue 70 ± 3.5)], black [BH (accession code Exp. line F8, hue 62.4 ± 1.7)], chartreuse [CH (accession code Exp, line F5, hue 2.3 ± 1.5)].

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    Heat map representation of log2-fold change in RNA expression of carotenoid and tocopherol-related genes and NAC transcription factors in tomato compared with the native red (RN) genotype. Tocopherol, chlorophyll, and carotenoid biosynthetic pathways adapted from Almeida et al. (2016) and Lira et al. (2016) are indicated in orange, green, and red, respectively. The genes (GGDR = geranylgeranyl reductase; HPPD = 4-hydroxyphenylpyruvate dioxygenase; PSY-1 = phytoene synthase 1; PSY-2 = phytoene synthase 2; CYC-B = chromoplast-specific lycopene β-cyclase; LCY-B = lycopene β-cyclase; DXS = 1-deoxy-D-xylulose phosphate synthase; VTE-5 = phytol kinase; VTE-2 = homogentisate phytyl transferase; MEP pathway = non-mevalonate pathway; PP/PDP = phytyl diphosphate; GGDP = geranylgeranyl diphosphate; MPBQ = 2-methyl-6-phytyl-1,4-benzoquinol; and DMBQ = 2,3-dimethyl-6-geranylgeranylbenzoquinol) are indicated by the color of the genotype that showed the greatest transcript levels (red, orange, or yellow): ON = orange native; YN = yellow native; PN = purple native; LRH = light red hybrid; LOH = light orange hybrid; CH = chartreuse hybrid; BH = black hybrid.

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    Principal component analysis showing the correlation between all studied parameters in tomato genotypes (ON = orange native; YN = yellow native; PN = purple native; LRH = light red hybrid; LOH = light orange hybrid; CH = chartreuse hybrid; BH = black hybrid); TEAC = Trolox-equivalent antioxidant capacity; GGDR = geranylgeranyl reductase; HPPD = 4-hydroxyphenylpyruvate dioxygenase; PSY-1 = phytoene synthase 1; PSY-2 = phytoene synthase 2; CYC-B = chromoplast-specific lycopene β-cyclase; LCY-B = lycopene β-cyclase; DXS = 1-deoxy-D-xylulose phosphate synthase; VTE-5 = phytol kinase; VTE-2 = homogentisate phytyl transferase.

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