Antioxidants, antioxidant capacity, and the expression of isoprenoid metabolism–related genes and two pigmentation-related transcription factors were studied in four native and four hybrid tomato (Solanum lycopersicum) genotypes with different-colored fruit. Red fruit genotypes were associated with greater lycopene, β-carotene, lipophilic antioxidant capacity, and greater chromoplast-specific lycopene β-cyclase (CYC-B) transcript levels. Orange fruit genotypes had greater concentrations of tocopherols and greater transcript levels of homogentisate phytyl transferase (VTE-2), 1-deoxy-D-xylulose phosphate synthase (DXS), and 4-hydroxyphenylpyruvate dioxygenase (HPPD). The yellow fruit genotype was greater in total polyphenol and hydrophilic antioxidant capacity with greater expression of geranylgeranyl reductase (GGDR), phytol kinase (VTE-5), phytoene synthase (PSY) 2, lycopene β-cyclase (LCY-B), SlNAC1, and SINAC4. Greater levels of individual antioxidants were associated with specific coloration of tomato fruit. Moreover, the negative correlations between the expression of PSY1 and VTE-5, and between lycopene and chlorophyll, suggest a balance between carotenoids, tocopherols, and chlorophylls. The results of this study support either the direct commercialization of tomatoes with different color fruit or use of their genotypes in breeding programs to increase antioxidant levels among existing cultivars.
We thank CONACYT (Consejo Nacional de Ciencia y Tecnología; scholarship 265752) and UAM (Universidad Autónoma Metropolitana) for its financial support.
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AlmeidaJ.AsísR.Noel MolineriV.SestariI.LiraB.S.CarrariF.PeresL.E.RossiM.2014Fruits from ripening impaired, chlorophyll degraded and jasmonate insensitive tomato mutants have altered tocopherol content and compositionPhytochemistry1117283
AlmeidaJ.AzevedoM.D.S.SpicherL.GlauserG.vom DorpK.GuyerL.del Valle CarranzaA.AsisR.Pereira de SouzamA.BuckeridgeM.DemarcoD.BresC.RothanC.PereiraL.E.HörtensteinerS.KesslerF.DörmannP.CarrariF.DemarcoD.2016Down-regulation of tomato PHYTOL KINASE strongly impairs tocopherol biosynthesis and affects prenyllipid metabolism in an organ-specific mannerJ. Expt. Bot.67919934
BaldetP.BresC.OkabeY.MauxionJ.P.JustD.BournonvilleC.MoriK.EzuraH.FerrandC.RothanC.2013Investigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutantsPlant Biotechnol.30309314
BarrosL.DueñasM.PinelaJ.CarvalhoA.M.BuelgaC.S.FerreiraI.C.2012Characterization and quantification of phenolic compounds in four tomato (Lycopersicon esculentum L.) farmers’ varieties in northeastern Portugal homegardensPlant Foods Hum. Nutr.67229234
BovyA.de VosR.KempeM.SchijlenE.PertejoM.A.MuirS.Santos-BuelgaC.2002High-flavonol tomatoes resulting from the heterologous expression of the maize transcription factor genes LC and C1Plant Cell1425092526
Di Paola NaranjoR.D.OtaizaS.SaragustiA.C.BaroniV.CarranzaA.D.V.PeraltaI.E.ValleE.M.CarrariF.AsisR.2016Hydrophilic antioxidants from Andean tomato landraces assessed by their bioactivities in vitro and in vivoFood Chem.206146155
LiraB.S.RosadoD.AlmeidaJ.de SouzaA.P.BuckeridgeM.S.PurgattoE.RossiM.2016Pheophytinase knockdown impacts carbon metabolism and nutraceutical content under normal growth conditions in tomatoPlant Cell Physiol.57642653
López-VidalO.CamejoD.Rivera-CabreraF.KönigsbergM.Villa-HernándezJ.M.Mendoza-EspinozaJ.A.de León-SánchezF.D.2016Mitochondrial ascorbate–glutathione cycle and proteomic analysis of carbonylated proteins during tomato (Solanum lycopersicum) fruit ripeningFood Chem.19410641072
Viuda-MartosM.Sánchez-ZapataE.Sayas-BarberáE.SendraE.Pérez-ÁlvarezE.Fernández-LópezJ.2014Tomato and tomato by products. Human health benefits of lycopene and its application to meat products: A reviewCrit. Rev. Food Sci. Nutr.5410321049
ZhangY.ButelliE.De StefanoR.SchoonbeekH.J.MagusinA.PagiaraniC.WellnerN.HillL.OrzaezD.GranellA.JonesJ.MartinC.2013Anthocyanins double the shelf life of tomatoes by delaying overripening and reducing susceptibility to gray moldCurr. Biol.2310941100
ZhuM.ChenG.ZhouS.TuY.WangY.DongT.Z.2014A new tomato NAC (NAM/ATAF1/2/CUC2) transcription factor, SlNAC4, functions as a positive regulator of fruit ripening and carotenoid accumulationPlant Cell Physiol.55119135