Variation in Ploidy and Karyological Diversity in Different Herbaceous Peony Cultivar Groups

in Journal of the American Society for Horticultural Science

The karyotypes of 21 herbaceous peony (Paeonia) cultivars were studied using root tip squashes revealing a wide variety of ploidy levels. There were three tetraploid (2n = 4x = 20), 11 triploid (2n = 3x = 15), and one diploid (2n = 2x = 10) cultivars in the hybrid group; five triploid (2n = 3x = 15) cultivars in the Itoh group; and one diploid (2n = 2x = 10) cultivar in the lactiflora group (LG). The asymmetry index (AI) ranged from 59.61% (‘Cytherea’) to 64.03% (‘Little Red Gem’). The karyotypes of all peony cultivars were 2A with 60% metacentric (m), 20% submetacentric (sm), and 20% subtelocentric (st) chromosomes. The karyotypic background of these 21 herbaceous peony cultivars is discussed in the context of the evolution of ploidy and the three cultivar groups. These results provide cytological information that would assist in a peony hybridization program.

Contributor Notes

This work was financially supported by the National Natural Science Foundation of China (31400591) and the Co-building Graduate Training and Development Program of the Beijing Municipal Commission of Education (BLCXY201615).

Corresponding author. E-mail: yuxiaonan626@126.com.

  • AnguloM.B.MassimilianoD.2009Karyotype analysis in eight species of Vernonia (Vernonieae, Asteraceae) from South AmericaCaryologia628188

    • Search Google Scholar
    • Export Citation
  • AranoH.1963Cytological studies in subfamily Carduoideae (Compositae) of Japan XIIIBot. Mag. Tokyo76419427

  • ChenR.Y.SongW.Q.LiX.L.LiM.X.LiangG.L.ChenC.B.2003Chromosome atlas of major economic plants genome in China (Tomus III): Chromosome atlas of garden flowering plants in China (translation). Science Press Beijing China (in Chinese)

  • DarkS.1936Meiosis in diploid and tetraploid Paeonia speciesGenetics32353372

  • DengT.MengY.SunH.NieZ.L.2011Chromosome counts and karyotypes in Chaetoseris and Stenoseris (Asteraceae-Cichorieae) from the Hengduan Mountains of SW ChinaJ. Syst. Evol.49339346

    • Search Google Scholar
    • Export Citation
  • DengX.L.HeX.J.HeW.L.GaoY.D.LiuH.Y.ZhangY.C.2009Karyotype and cytogeography of the genus Heracleum (Apiaceae) in the Hengduan MountainsPlant Syst. Evol.47273285

    • Search Google Scholar
    • Export Citation
  • DingK.Y.LiuM.Y.1991Taxonomic studies on the so-called Paeonia obvata from northeast ChinaBull. Bot. Res.1128590(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • GaoY.D.ZhouS.D.HeX.J.WanJ.2012Chromosome diversity and evolution in tribe Lilieae (Liliaceae) with emphasis on Chinese speciesPlant Res.1255569

    • Search Google Scholar
    • Export Citation
  • GongX.GuQ.J.WuQ.A.1991Cytological study of seven populations in Paeonia delavayi var. luteaActa Bot. Yunan13402410(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • GongX.XiaoD.J.GuZ.J.LuY.X.1999Giemsa C - banding patterns in 8 populations of Paeonia delavayi var. luteaActa Bot. Yunan21477482(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • GrantV.1981Plant speciation. Columbia Univ. Press New York NY

  • HaoL.H.MaH.Teixeira da SilvaJ.A.YuX.N.2016Pollen morphology of herbaceous peonies with different ploidy levelsJ. Amer. Soc. Hort. Sci.141275284

    • Search Google Scholar
    • Export Citation
  • HongD.Y.PanK.Y.ZhouS.L.WangX.Q.ZhangD.M.LuoY.B.RaoG.Y.MaL.M.2010Peonies of the world. Royal Botanic Gardens Kew Richmond UK

  • HongD.Y.ZhangZ.K.ZhuX.Y.1988The study of Paeonia (1) - Karyotypic study of several wild species in PaeoniaJ. Syst. Evol.263343(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • HouX.G.DuanC.Y.LiuS.Y.LvJ.X.ZhangY.B.LiJ.J.2006Advances on chromosome study of Chinese tree peonyChinese Agr. Sci. Bul.22307309(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • JiL.J.Teixeira da SilvaJ.A.ZhangJ.J.TangZ.J.YuX.N.2014Development and application of 15 novel polymorphic microsatellite markers for sect. Paeonia (Paeonia L.)Biochem. Syst. Ecol.54257266

    • Search Google Scholar
    • Export Citation
  • JianH.Y.ZhangH.ZhangT.LiS.F.WangQ.G.YanH.J.QiuX.Q.TangK.X.2010Karyotypes analysis of different varieties on Rosa odorata sweetJ. Plant Genet. Resources11457461(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • JianH.Y.ZhangT.WangQ.G.LiS.B.ZhangH.TangK.X.2013Karyological diversity of wild Rosa in Yunnan, southwestern ChinaGenet. Resources Crop Evol.60115127

    • Search Google Scholar
    • Export Citation
  • KuoS.R.WangT.T.HuangT.C.1972Karyotype analysis of some Formosan gymnospermsTaiwania176680

  • La CourL.F.1952Chromosome counts of species and varieties of garden plantsJ. Ann. Rpt. John Innes Hort. Inst.2383110

  • LevanA.FredgaK.SandbergA.A.1964Nomenclature centromeric position on chromosomesHereditas52201220

  • LevinD.A.2002The role of chromosomal change in plant evolution. Oxford Univ. Press New York NY

  • LiM.X.ZhangX.F.1982The study of cytogenetic triploid tree peony (translation)Hereditas41921(in Chinese)

  • LiY.Z.XuJ.L.1990A comparative karyotypic study of three cultivars in Paeonia. J. Anhui Univ. (Natural Sci.) 14:90–94 (in Chinese with English abstract)

  • LiuJ.X.2016Compatibility of distant hybridization in Paeonia L. and identification of F1 hybrid progenies. MSc Diss Beijing Forestry Univ. Beijing China (in Chinese with English abstract)

  • MaH.YuX.N.2013Studies on karyotypes of four cultivars in herbaceous peonyAgr. Univ. Hebei366165(in Chinese with English abstract)

  • PaszkoB.2006A critical review and a new proposal of karyotype asymmetry indicesPlant Syst. Evol.2583948

  • QinK.J.2004Paeonia. China Forestry Publ. House Beijing China (in Chinese with English abstract)

  • SangT.PanJ.ZhangD.M.DianeF.WangC.PanK.Y.HongD.Y.2004Origins of polyploids: An example from peonies (Paeonia) and a model for angiospermsBiol. J. Linn. Soc. Lond.82561571

    • Search Google Scholar
    • Export Citation
  • ShenJ.S.YeY.J.PanH.T.ZhangQ.X.2015Karyotype analysis of twelve Forsythia species and cultivarsPlant Genet. Resour.16178184(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • StebbinsG.L.1971Chromosomal evolution in higher plant. Edward Arnold London UK

  • SunJ.F.2007Preliminary report on the introduction of intersectional hybrids between tree and herbaceous peoniesChinese Landscape Architecture235154(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • WangS.Q.2010Advances on chromosome study of section Paeonia in ChinaNorth Hort.16217219(in Chinese with English abstract)

  • WuG.X.CuiL.H.LiuS.H.YaoF.SiS.X.RenS.H.2011Preliminary studying report of cultivating and demonstrating Itoh hybrids introduced from abroadNorth. Hort.246771(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • YangC.T.FanX.WangY.ShaL.N.ZhangH.Q.KangH.Y.ChenQ.WuD.D.ZhouH.2014Karyotype studies on 12 populations of Leymus secalinus (Georgi) Tzvelev (Poaceae: Triticeae)Caryologia67110115

    • Search Google Scholar
    • Export Citation
  • YangD.Q.ZhuX.F.1989Karyotypic studies of Paeonia obvata, P. delavayi and P. delavary var. luteaPlant Diversity Evol.11139144(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • YangL.H.YuX.N.2016Phylogenetic relationship analysis of different cultivars groups of herbaceous peony according morphological characteristicsJ. Plant Genet. Resources17209216(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • YangL.H.YuanY.B.YuX.N.2015Study on the introduction and adaptability of herbaceous peony of hybrid group p. 168–175. In: Advances in ornamental horticulture of China. China Forestry Publ. House Beijing China (in Chinese with English abstract)

  • YuX.N.YuanQ.L.SongH.Z.2011Comparison on the cultivation and application histories of herbaceous peony and its flower cultures in China and Western Countries. JChinese Landscape Architecture277781(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • ZhangD.C.ShaoJ.Z.2000Karyotypes analysis of four species of Paeonia L. from AnhuiJ. Anhui Normal Univ. (Natural Sci.)23327330(in Chinese with English abstract)

    • Search Google Scholar
    • Export Citation
  • ZhangY.ZhuM.L.DaiS.L.2013Analysis of karyotype diversity of 40 Chinese chrysanthemum cultivarsJ. Syst. Evol.51335352

  • ZhaoN.2014Study on morphology and chromosome karyotype of distant hybridization progenies and their parents of tree peony. MSc Diss. Beijing Forestry Univ. Beijing China (in Chinese with English abstract)

All Time Past Year Past 30 Days
Abstract Views 186 186 13
Full Text Views 40 40 1
PDF Downloads 10 10 0