Cytogenetic Analysis of Lilium rosthornii

in Journal of the American Society for Horticultural Science

Lily (Lilium L.) species produce among the most important cut flowers worldwide. China has ≈55 species of Lilium. Although many plants from this genus have been used in hybridization efforts, their cytology has remained unclear. The goal of the current study was to characterize the chromosomes of Lilium rosthornii Diels. Root tips were used to characterize Giemsa C-banding, propidium iodide (PI) banding, and 45S rDNA locations. The karyotype of L. rosthornii belongs to type 3B. C-banding revealed polymorphic banding patterns with the following formula: 2n = 24 = CI = 4C + 14CI+ + 2I+ +2I+ 2. Two of the four 45S rDNA hybridization sites were located at pericentromeric positions on the two short arms of the homologues of chromosome 1, and the other two were located on the long arms of one chromosome 6 homolog and one chromosome 11 homolog. Six of the eight PI bands were detected in the centromeres of the homologues of chromosomes 1, 5, and 8, and the other two PI bands were detected on the long arms of one chromosome 6 and one chromosome 11. Lilium rosthornii showed enriched banding in both Giemsa C-banding and PI painting. Interestingly, not all 45S rDNA was located in homologous chromosomal locations. These results may provide reference data for L. rosthornii for use in further Lilium breeding.

Contributor Notes

This work was supported by the “948” project of the State Administration of Forestry of China (2013-4-34), Jiangsu Provincial Key Basic Research foundation for Universities (16KJA220001), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

We thank Chen Peidu and Wang Xiu’e from Nanjing Agricultural University and to Yuan Shenghua from Yale University for their suggestions and help with the experiments and with writing the paper.

Corresponding author. E-mail: ximenglinjfu@126.com.

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Article Figures

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    Flowers of Lilium rosthornii collected from Nanchuan, Chongqing Province, China (lat. 29°08′17″N, long. 107°10′40″E). The flower characters included rolled petals, yellow or yellow red color, and scattered purple-red spots.

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    C-banding, propium iodide (PI) painting, and 45S rDNA fluorescent in situ hybridization (FISH) analysis of Lilium rosthornii. (A) Chromosome karyotype of L. rosthornii chromosomes based on C-banding. Arrows of the same color indicate a pair of homologous chromosomes. The two white arrows indicate the secondary constrictions. (B) PI painting of L. rosthornii chromosomes. Eight red PI bands can be detected on different chromosomes. (C) 45S rDNA FISH analysis of L. rosthornii chromosomes. Four 45S rDNA hybridization sites (green) can be detected. Two sites are located on the short arms of the two longest chromosomes. The other two sites are located on the long arms of two nonhomologous chromosomes. Bar = 10 μm.

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    Comparisons of homologous chromosomes in Lilium rosthornii using different methods. (A) C-banding karyotype of L. rosthornii. Homologues chromosomes showed similar banding patterns, while different patterns were observed between different pairs of homologous chromosomes. (B) C-banding pattern of L. rosthornii. (C) Propium iodide (PI) painting of L. rosthornii. Eight PI bands were detected, at the centromeres of homologous chromosomes pairs 1, 5, and 8 and on the long arms of one chromosome 6 homolog and one chromosome 11 homolog. (D) 45S rDNA fluorescent in situ hybridization analysis of L. rosthornii. Two of the four 45S rDNA hybridization sites were located at pericentromeric positions on the short arms of the chromosome 1 homologs, and the other two sites were located on the long arms of one chromosome 6 homolog and one chromosome 11 homolog.

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