Identifying Crabapple Cultivars by Isozymes

in Journal of the American Society for Horticultural Science

Forty-five crabapple (Malus spp.) cultivars were evaluated for 16 isozyme systems by starch gel electrophoresis. Of the 16 systems evaluated, 6 were useful in separating among cultivars. Enzyme systems used to distinguish among the cultivars included alcohol dehydrogenase, aspartate aminotransferase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucoisomerase, and shikimate dehydrogenase. Each enzyme system produced one well-resolved polymorphic region except for 6-phosphogluconate dehydrogenase, which produced two. Most crabapple selections could be identified when all six enzymes were evaluated. Alcohol dehydrogenase had the most diagnostic banding patterns useful for cultivar identification.

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