Randomly Amplified Polymorphic DNAs in the Cultivated Strawberry, Fragaria ×ananassa

in Journal of the American Society for Horticultural Science

Eight strawberry cultivars or advanced selections from the Univ. of California, Davis, breeding program were screened for polymorphisms using the polymerase chain reaction (PCR) and 43 random 10-base DNA primers. Over 60% of the primers screened resulted in replicable polymorphic banding patterns (amplification profiles), and a subset of ten primers that exhibited high levels of amplification profile polymorphism was used to identify each of the eight genotypes uniquely. There was also a significant product-moment correlation (r = 0.64, P < 0.01) between number of shared amplification profile phenotypes and pairwise coefficient of coancestry. This technology shows high promise as a means of verifying the identity of cultivars and developing a genetic map of the octoploid cultivated strawberry.

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