Variations in African Violet `Crimson Frost' Micropropagated by Homogenized Leaf Tissue Culture

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  • 1 Department of Horticulture, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
  • 2 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.

Adventitious buds regenerated from homogenized leaf tissue of Saintpaulia ionantha Wendl. `Crimson Frost' were micropropagated to determine types and frequencies of the variants obtained. Plants grown for one year in a greenhouse showed 67% variation and 33% normality. A higher rate of variation was observed in leaf color rather than in leaf shape. The variations in leaf color and leaf shape were 67% and 19%, respectively. In regard to flower type, greater numbers of semidouble and double types were obtained as compared to single types. Both flower types showed a much higher rate of normal (mixed) color (81%) as compared to pink, red or white (19%). An sodium dodecylsulfate polyacrylamide gel electrophoresis profile of protein extracted from leaves of the stock plants and the variants indicated no difference between them and did not reflect the variation in phenotype.

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