Laboratory Techniques for Determining Ploidy in Plants

in HortTechnology
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  • 1 Department of Horticulture and Agronomy, MSC 3Q, Box 30003, New Mexico State University, Las Cruces, NM 88003-0003.

Determination of ploidy is an essential plant breeding technique. Laboratory exercises for teaching students how to determine ploidy in plant tissues using various techniques are described for geranium and onion. The different methods include root tip squashes, pollen mother cell squashes, pollen grain size and germinal pore counts, stomata size and density determination, and gross morphology.