Laboratory Exercises Illustrating Organogenesis and Transformation using Chrysanthemum Cultivars

in HortTechnology
Authors: R.N. Trigiano1 and R.A. May2
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  • 1 Institute of Agriculture, Department of Ornamental Horticulture and Landscape Design, The University of Tennessee, Knoxville, TN 37901-1071.
  • | 2 Department of Horticulture, Michigan State University, East Lansing, MI 48824-1325.

A tissue culture laboratory exercise illustrating regeneration of whole plants from leaf segments of chrysanthemum by organogenesis is described. Using simple, common media, shoots can be generated in 5 weeks and rooted after an additional 3 weeks. Acclimatization of plants can be accomplished in a simple mistbed in the greenhouse. The exercise is adaptable to depict genotype differences among cultivars, optimization of shoot induction, effects of growth regulators, and experimental design. Callus is typically not formed during shoot formation; however, co-cultivation of leaf segments with a virulent strain of Agrobacterium tumefaciens produces callus with a strain of disarmed A. tumefaciens harboring NPTII construct affects regeneration of plants resistant to kanamycin.

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