The Use of Fogging and Phototron Units to Acclimatize in Vitro-derived Apple Shoots

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  • 1 Department of Horticulture. 2.58 Plant and Animal Biotechnology laboratory University of Illinois, 1201 Gregory Drive, Urbana, IL 61801.

Acclimatization and growth of in vitro-derived apple shoots of two apple scion apple cultivars were compared under fogged conditions in a greenhouse and in a commercial growth cabinet (Phototron). Plant survival rates of microcuttings of `Royal Gala' and `Jonagold' were significantly better when maintained in the Phototron units than when grown in a greenhouse under fog. The number and length of roots on microcuttings was significantly higher in the Phototron than under fog. In the present study, we demonstrated that the Phototron environment was better than a fogged greenhouse for establishing apple shoots ex vitro. However, the Phototron units are so small that they hold no more than 100 to 120 plants at a time. Therefore, the units will be of most value to growers or individuals in laboratories who do not have a constant need for acclimatization facilities. Growers who acclimatize many plants should continue to use fogging or misting facilities.

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