Taiwanese wild grape is a perennial herb that is traditionally used in medical therapy in eastern Asia. Recently, growers tried to cultivate taiwanese wild grape artificially, but they had some problems such as low rooting percentage in clonal propagation. In a preliminary study in 2003, we examined the rooting percentage of clonal hardwood cuttings of taiwanese wild grape using conventional materials. However, the rooting percentage of taiwanese wild grape hardwood cutting was too low to be useful for mass propagation. It was reported that taiwanese wild grape could be clonal propagated through high-frequency shoot tip culture (Lu, 2005). However, the proliferation system takes lots of labor and time. Some reports mentioned that softwood cuttings could be used for clonal propagation of norton grapes (Vitis aestivalis) and muscadine grapes (Vitis rotundifolia) (Goode and Lane, 1983; Keeley et al., 2003). So we tried to take softwood cuttings instead of hardwood cuttings for clonal propagation.
For softwood cuttings, many researchers have focused on the atmospheric environment of the propagation area to promote rooting percentage. Since the 1950s, overhead intermittent mist irrigation has been the conventional means of maintaining the water potential of cuttings and it has increased the rooting percentage in many plant species (LeBude et al., 2004; Mudge et al., 1995). Subirrigation is another common system used to reduce water stress for successful rooting of cuttings (Owen et al., 2003; Post, 1946; Zhang et al., 1997). However, these two systems are costly in terms of equipment. Therefore, we built a floating culture system (Fig. 1) for clonal propagation of taiwanese wild grape.
The objective of this study was to examine the effects of cutting type, medium type, and auxin concentration on the rooting percentage of taiwanese wild grape in a floating culture system.
Burholt, D.R. & Van't Hof, J. 1971 Quantitative thermal-induced changes in growth and cell population kinetics of Helianthus roots Amer. J. Bot. 58 386 393
Dykeman, B. 1976 Temperature relationship in root initiation and development of cuttings Comb. Proc. Intl. Plant Prop. Soc. 26 201 207
Keeley, K., Preece, J.E. & Taylor, B.H. 2003 Increased rooting of ‘Norton’ grape cuttings using auxins and gibberellin biosynthesis inhibitors HortScience 38 281 283
Komissarov, D.A. 1968 Biological basis for the propagation of woody plants by cutting. Israel Program for Scientific Translations. IPST, Jerusalem
LeBude, A.V., Goldfarb, B., Blazich, F.A., Farrell, W.C. & Frampton, J. 2004 Mist, substrate water potential and cutting water potential influence rooting of stem cuttings of loblolly pine tree Tree Physiol. 24 823 831
Lu, M.C. 2005 Micropropagation of Vitis thunbergii Sieb. Et Zucc., a medicinal herb, through high-frequency shoot tip culture Scientia Hort. 107 64 69
Mudge, K.W., Mwaja, V.N., Itulya, F.M. & Ochieng, J. 1995 Comparison of four moisture management systems for cutting propagation of bougainvillea, hibiscus and kei apple J. Amer. Soc. Hort. Sci. 120 366 373
Owen J.S. Jr, Johnson, W.A. & Maynard, B.K. 2003 Effects of auxin concentration and medium temperature on four woody plant taxa propagated by stem cuttings using recirculating subirrigation in a growth chamber J. Environ. Hort. 21 126 130
Robert, M.J., Frampton, J. & Hain, F.P. 2005 Vegetative propagation of mature eastern and carolina hemlocks by rooted softwood cuttings HortScience 40 1469 1473
Sabir, A., Kara, Z., Kucukbasmact, F. & Yucel, N.K. 2004 Effects of different rooting media and auxin treatments on the rooting ability of Rupestris du Lot (Vitis rupestris) rootstock cuttings J. Food Agr. Environ. 2 307 309
Singh, S.K., Khawale, R.N. & Singh, S.P. 2004 Technique for rapid in vitro multiplication of Vitis vinifera L. cultivars J. Hort. Sci. Biotechnol. 79 267 272
Soffer, H. & Burger, D.W. 1988 Effects of dissolved oxygen concentrations in aero-hydroponics on the formation and growth of adventitious roots J. Amer. Soc. Hort. Sci. 113 218 221
Zhang, H., Graves, W.R. & Townsend, A.M. 1997 Water loss and survival of stem cuttings of two maple cultivars held in subirrigated medium at 24 to 33 °C HortScience 32 129 131