In vivo Micrografts in Almond and Their Application in Breeding Programs

in HortTechnology
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  • 1 Department of Pomology, University of California, 1 Shields Avenue, Davis CA 95616.

The techniques of micrografting provide useful tools for the early propagation of breeding lines in variety improvement programs. In this study, the success of various in vivo micrografts of buds of `Nonpareil' almond (Prunus dulcis) seedlings have been examined. Variables tested include type of microscion, the rootstock genotype, and the growth stage of the rootstock. Microscions tested included small [3 mm (0.12 inches)] microwedges from either unsprouted or recently sprouted buds. Approach micrografts were also evaluated. Three-month-old woody rootstocks tested included `Nemaguard' and `Nemared' peach (P. persica) rootstocks, and the `Hansen' (peach × almond) hybrid rootstocks. `Nemared' rootstocks were also grafted after 1 month of growth when the tissue was still herbaceous. Micrografts onto woody rootstocks and using recently sprouted buds showed the best results. With very young or weak seedlings, where the recovery of microscions was difficult, the use of approach micrografts onto herbaceous rootstock proved a useful technique.

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