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The Development of Paeonia ostii Seeds and Oil Quality Formation

Authors:
Yuting Zou Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Yanan Wang Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Mingwei Zhu Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Shuxian Li Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Qiuyue Ma Institute of Leisure Agriculture in Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

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Abstract

Paeonia ostii is a woody oil crop with potential value as an edible oil. With the aim of acquiring systematic knowledge of the development of P. ostii seeds, the oil content, biomass, and water content of the seeds were determined. Changes in the distribution of hydrogen protons in P. ostii seeds during follicle development were traced using magnetic resonance imaging (MRI). The formation of oil bodies in the endosperm and embryo was observed using transmission electron microscopy (TEM). Dynamic changes in oleic acid, linoleic acid, and α-linolenic acid contents were assessed by gas chromatography-mass spectrometry (GC-MS). The magnetic resonance images showed that, during early follicle development [45–85 days after flowering (DAF)], a greater quantity of liquid mucus was present within the seeds, and seeds in the same follicle developed at different rates. At 95 to 115 DAF, proton density was distributed evenly in all areas of the seed. A small dark area appeared in the center of the seed, and mucus in the follicles and water in the pericarp disappeared gradually. TEM observations showed that at 45 DAF, a few oil bodies were scattered at the cell periphery in the endosperm, and oil bodies were more numerous in the embryo. With the progression of seed development, the number and size of oil bodies in the embryo and endosperm continued to increase. The fresh and dry mass of P. ostii seeds increased from 45 to 105 DAF, then decreased after 105 DAF. The moisture content decreased, whereas the oil content increased and attained 33.1% at seed maturity. The three predominant unsaturated fatty acids accumulated simultaneously and showed stages of initial accumulation (45–65 DAF) and rapid accumulation (65–105 DAF). The results suggest that 65 to 105 DAF is a critical period for unsaturated fatty acid accumulation in P. ostii seeds.

Seeds serve as a strong metabolic library, which is why seed development is an important stage for higher plants. During the process of seed maturation, substances such as starch, protein, and lipids are transformed and accumulated. Pavithra et al. (2014) observed that oil content in Pongamia pinnata seeds increased with seed maturation, and oleic acid content determined by GC-MS remained high at the mature stage. Yuan et al. (2015) reported that palmitic acid, stearic acid, and oleic acid contents of Camelina sativa seeds are high during the early stage of seed development, but the oil content is low. However, the oil content in the seeds accumulated rapidly during the intermediate stage of seed development and increased further during the final stage of seed development. Zhang et al. (2017) reported a linear decline in water content during seed development in Styrax tonkinensis. They also observed that oil accumulation fluctuated throughout seed development, and the contents of oleic acid and linoleic acid increased during the early stage of seed development. In contrast, oleic acid content increased slowly and linoleic acid content decreased slightly during the final stage of seed development.

Paeonia suffruticosa, the tree peony, is a famous traditional flower in China (Cheng and Yu, 2008). Paeonia ostii, commercially referred to as ‘Fengdan’, is a perennial deciduous shrub belonging to section Moutan of Paeonia, and is the most widely used species because of the high oil content of the seeds, 90.0% of which is unsaturated fatty acids (UFAs). Among the UFAs, α-linolenic acid is the predominant type, accounting for 42.7% of the total content, which is substantially greater than that of traditional oil crops, such as soybean, rapeseed, peanut, corn, and sunflower (Li et al., 2014). For this reason, peony seed oil is being developed as a novel source of woody edible oil and is widely produced in China. In addition, the flower of P. ostii has a unique taste and high nutritional value (Gai et al., 2013; Han et al., 2011; Yuan et al., 2011). P. ostii is also of considerable medicinal value. The root bark is a traditional Chinese medicine rich in nutrients and it has therapeutic effects. Because of the wide variety of uses, P. ostii has received increasing attention from the Chinese government for development as an emerging woody oil crop (Li, 2014).

Currently present, research on peony is focused predominantly on the flower color and oil contents. However, relevant research on the behavior of seed oil accumulation and changes in UFAs during seed development are extremely scarce. In our study, we investigated seed and follicle development of P. ostii ‘Fengdan’ using MRI. The formation of oil bodies was observed using TEM. The seed mass, together with the contents of water, oil, α-linolenic acid, oleic acid, and linoleic acid in the seeds at different developmental stages were determined. Our aim was to acquire systematic knowledge of oil body formation and accumulation of UFAs during seed maturation, which is essential to the management of P. ostii individuals in cultivation to optimize seed oil content. Optimal management will not only improve P. ostii seed oil quality, but also increase the seed yield to a certain extent (Shi, 2016).

Materials and Methods

Plant material.

We marked 100 six-year-old trees of P. ostii for collection of fruit samples, which were propagated from the seeds of Heze, Shandong Province, China (lat. 35°14′N, long. 115°26′E). The trees were planted in the field of the Nanjing Green Universe Peony Science & Technology Co. Ltd, Jiangsu Province, China (lat. 32°11′–32°27′N, long. 118°34′–119°03′E), with 60-cm spacing between plants and 80-cm spacing between rows. This area belongs to the subtropical, monsoon climate zone, with mild climate and four distinctive seasons. The annual average precipitation, temperature, and accumulated temperature of the experimental site are 1004.4 mm, 16 °C, and 4820 °C, respectively.

All chosen individuals bore five subbranches, blossomed on the same day of 6 Apr. 2019, and the flower sizes were almost identical. Forty tagged follicles on the marked trees were collected randomly at 20-d intervals from 45 to 85 DAF, and then at 10-d intervals until follicle maturity. After collection, a complete fresh fruit with five follicles was used for MRI analysis. About 80 seeds were extracted manually from the remaining follicles for measurement of seed biomass and analysis of moisture and oil contents, as well as TEM observation. The extracted seeds were stored in sealed, zippered plastic bags at –80 °C.

MRI analysis.

MRI was performed using a MesoMR23-60H-I NMR imager (Suzhou Niumag Company Ltd, Suzhou, China) with a resonance frequency of 23.423 MHz, a probe coil diameter of 60 mm, and a magnet temperature of 32.00 ± 0.01 °C. For all images, fresh follicles were used and the acquisition parameters were as follows: field of view, 100 × 100 mm; repetition time, 500 ms; echo time, 20 ms; slice thickness, 13.6 mm; number of sampling times, 32; and matrix, 256 × 256.

Oil body observation by TEM.

Fresh seeds from each sample were used to examine oil body formation using TEM. The seeds were cut vertically. Samples of the endosperm and embryo were prepared following the method described by Zhang et al. (2018), with some modifications. Briefly, the samples were fixed immediately with 2.5% (v/v) glutaraldehyde in sodium phosphate buffer (pH 7.2). The samples were postfixed in osmium tetroxide and dehydrated in ethyl alcohol at 4 °C. The samples were embedded in Spurr’s resin and observed at 60 kV with a JEM-1200 transmission electron microscope (JEOL, Tokyo, Japan).

Seed moisture content, and fresh and dry weight analysis.

The fresh and dry weight, as well as the moisture content of the seeds were estimated for three replicates of 20 seeds. The seeds were cut into small pieces of less than 2 mm and were weighed to 0.001-g accuracy to obtain the fresh weight for each harvest. The seeds were then placed in a freeze-dryer (Christ Alpha 1-4 LOC-1; Martin Christ, Osterode, Germany) with a pressure of 0.001 MPa at an initial temperature of –76 °C, followed by precooling at –80 °C for 2 h. After lyophilization for 24 h, the samples attained a constant weight. The samples were then placed in a desiccator with silica gel. After the samples had returned to room temperature, they were reweighed to determine the dry weight. The moisture content at each sampling time point was calculated and expressed as the percentage weight loss relative to the initial weight.

Determination of seed oil content.

Seed oil was extracted in accordance with the method described by Zhang et al. (2017). Twenty seeds with three replicates at each developmental stage were cut into small pieces and freeze-dried at –76 °C until a constant mass was attained. The tissue pieces were ground to a powder and 1 g of powder was weighed. The oil was extracted with petroleum ether (30 to 60 °C) using a Soxhlet apparatus as described by Dutta et al. (2014). The flask temperature was maintained at 60 °C and the samples were extracted for 96 h. After extraction, the powder was reweighed, and the amount of oil was expressed as the percentage weight loss relative to the initial dry weight per gram.

Measurement of UFA content by GC-MS.

This experiment mainly followed the steps outlined by Zarringhalami et al. (2011), with some modifications. Fatty acid methyl esters were prepared by placing oil samples (0.2 g) extracted at different stages of seed development in screw-cap vials; 4 mL methanolic sodium hydroxide (0.5 mol/L) was added to each vial. The vials were sonicated with an ultrasonic cleaner (KQ5200; Kunshan Ultrasonic Instrument Company, Suzhou, China) in a water bath at 60 °C until the oil droplets were dissolved completely. The vials were removed from the water bath and allowed to cool to room temperature. Next, 2 mL of 14% boron trifluoride was added to the vial as a catalyst, and the vials were sonicated in a water bath at 60 °C for 10 min. After cooling to room temperature, saturated sodium chloride solution (2 mL) and hexane (2 mL) were added to the vials. The vials were corked and shaken thoroughly, then allowed to rest for 5 min. The upper hexane layer containing the methyl esters was collected and injected into a gas chromatograph (GC; Unicam 4600, Shimadzu, Kyoto, Japan).

The contents of oleic acid, linoleic acid, and α-linolenic acid were determined using a GC equipped with a flame ionization detector (FID). The GC-MS conditions were as follows. A fused-silica capillary column (30-m × 0.25-mm i.d. × 0.25-μm film thickness) (TR-WAXMS; Thermo Scientific, Waltham, MA) was used. The GC split ratio was 1:10, and samples of about 0.5 μL were injected. The FID and injector temperatures were set to 230 °C and 250 °C, respectively. The initial column temperature was maintained at 80 °C for 2.5 min and increased to 210 °C at a rate of 15 °C/min and was held at 210 °C. The temperature was then increased at a rate of 2 °C/min until it attained 230 °C and was held for 10 min. Helium was used as the carrier gas at a column head pressure of 20 psi. The sample peaks were identified by comparison with retention times for a pure standard mixture (Aldrich Chemical Company, Milwaukee, WI). The peak areas were calculated and the percentages of the areas were determined.

Statistical analysis.

Descriptive statistics were derived using Excel 2010 (Microsoft Corporation, Redmond, WA). One-way analysis of variance was performed using IBM SPSS Statistics 22.0 (IBM Corporation, Armonk, NY) followed by Duncan’s multiple range test for comparison of all pairs of treatment means. The P values were considered to be significant within groups at the 0.05 significance level. The magnetic resonance images were analyzed using OsiriX Lite software (version 7.0.4; Pixmeo, Geneva, Switzerland) and Adobe Photoshop 7.0 (Adobe, San Jose, CA).

Results

Fresh and dry biomass of seeds.

The fresh weight of P. ostii seeds increased rapidly from 45 to 65 DAF (Fig. 1). From 65 to 105 DAF, the rate of increase in fresh weight declined slightly, but it maintained a linear upward trend, attaining a maximum fresh weight of 10.6 g (20 seeds, 105 DAF). Subsequently, a decline in fresh weight was observed at 115 DAF.

Fig. 1.
Fig. 1.

Fresh and dry weight of Paeonia ostii seeds during follicle maturation. The different lowercase letters are significantly different according Duncan’s multiple range test at P ≤ 0.05.

Citation: HortScience horts 55, 8; 10.21273/HORTSCI15074-20

The dry weight showed a similar pattern to that of the fresh weight, with the weight increasing from 45 to 105 DAF. The maximum dry weight was 6.0 g at 105 DAF, from an initial weight of 1.1 g. After 115 DAF, the dry weight decreased, but the decline was less remarkable than that observed for the fresh weight (Fig. 1).

Water and oil contents.

The water and oil contents showed opposite trends during the development of P. ostii seeds (Fig. 2). The water content decreased significantly from 74.3% to 46.5% until 95 DAF. Subsequently, the water content maintained a slow decrease from 43.1% (105 DAF) to 41.6% (115 DAF); the decline was not significant. It is worth noting that 41.6% water content is extremely high compared with that of seeds of a majority of plant species.

Fig. 2.
Fig. 2.

Water and oil contents of Paeonia ostii seeds during follicle maturation. The different lowercase letters are significantly different according Duncan’s multiple range test at P ≤ 0.05.

Citation: HortScience horts 55, 8; 10.21273/HORTSCI15074-20

The oil content showed a rapid increase from 45 to 85 DAF—from 13.8% to 30.1%. From 85 to 105 DAF, the oil content increased slightly. At 115 DAF, the oil content showed an abrupt increase, attaining the maximum value of 33.1%.

MRI analysis of follicle development.

MRI is capable of visualizing internal structural information to reflect the state of hydrogen protons (which are predominantly associated with water and oil in the samples) in a nondestructive manner (Prestes et al., 2007). The principle of MRI is that it uses static magnetic fields and radio frequencies to obtain images of proton mobility in biological systems. For a grayscale image, the greater the density of the hydrogen protons, the brighter the image (Mou et al., 2016). For a pseudocolor image, the color of each voxel represents the proton density, and colors from blue to bright yellow to red represent increased amounts of hydrogen protons in the tissues. The number of seeds in individual follicles, detected by MRI, differed considerably, varying from 4 to 13. The proton density images of the developing P. ostii seeds also emphasize the area of interest where the water and oil protons are present.

At 45 DAF (Fig. 3A), the brightest region of the seed was the peripheral area near the seedcoat, whereas the central area of the endosperm was dark. The size of dark areas in the same follicle varied. Within the follicles, the locule was filled predominantly with liquid mucus, which was further confirmed by peeling the follicles. From the pseudocolor image (Fig. 3B), it was observed that the peripheral area of some seeds was bright red, but yellow for other seeds. The color of the central area was green. This led us to conclude that the proton density in the seed was greatest near the seedcoat and that seeds in the same follicle did not develop at the same rate. At 65 DAF (Fig. 3C), the entire seed was brighter than at 45 DAF and the dark space in the central area of the seed disappeared. However, a considerable volume of mucus was still present within the locule of the follicle. Based on the pseudocolor image (Fig. 3D), different colors were observed in different areas of the seed, and the developmental stage of the seeds still varied. At 85 DAF (Fig. 3E), the brightness of the pericarp was less than observed previously and the seed shape had become irregular as a result of squeezing of the seeds, accompanied by a marked decrease in the amount of liquid mucus in the follicle. The internal development of the seed was completed at this stage. With maturation of the seeds (95 DAF), red areas were detected in almost all seeds in the follicles in the pseudocolor image (Fig. 3H), showing that proton density in P. ostii follicles had increased again. At 105 DAF (Fig. 3I), a small dark area (as shown by the red arrow) was observed in the center of the seed, and mucus in the follicle had completely disappeared. At 115 DAF (Fig. 3K), the coat of P. ostii follicles in some areas was extremely dark and the outline of the pericarp was indistinct, indicating that little moisture was present in the pericarp. Some follicles sampled at this time had dehisced and the seeds were fully ripe.

Fig. 3.
Fig. 3.

Magnetic resonance images of Paeonia ostii follicles during maturation. Grayscale and pseudo-color images of follicles (A, B) 45 d after flowering (DAF), (C, D) 65 DAF, (E, F) 85 DAF, (G, H) 95 DAF, (I, J) 105 DAF, and (K, L) 115 DAF. The color scale in the legend represents, from deep red to yellow to deep blue, a gradual decrease in proton density.

Citation: HortScience horts 55, 8; 10.21273/HORTSCI15074-20

TEM observation of oil bodies.

In this study TEM was used to trace the formation and accumulation of oil bodies in the endosperm and embryo (Fig. 4). At the early stage of seed development (45 DAF), the cell wall boundaries were distinct and the intercellular spaces were large. A large number of vacuoles were detected that occupied a considerable volume of the cell lumen. Only a few oil bodies were observed, and they were scattered at the periphery of the cell wall and vacuoles. At 65 DAF, the number of oil bodies had increased substantially. The oil bodies were scattered throughout the cell, but still occupied a relatively small volume. Many spaces were present among the oil bodies. Highly electron-dense substances were gathered into a relatively large mass and were surrounded by the oil bodies, which were variable in size. At this time, the vacuoles disappeared. At 85 DAF, the sizes of oil bodies increased and the spaces between the oil bodies were reduced in size. By the time of P. ostii seed maturity (115 DAF), a large amount of tightly packed oil bodies almost filled the spaces between the highly electron-dense substances.

Fig. 4.
Fig. 4.

Ultrastructure of endosperm and embryo cells of Paeonia ostii seeds at 45, 65, 85, and 115 d after flowering (DAF) visualized by transmission electron microscopy. OB, oil body; BG, highly electron-dense substance; CW, cell wall; N, nucleus.

Citation: HortScience horts 55, 8; 10.21273/HORTSCI15074-20

Compared with the images of the endosperm, no notable variation was observed in the images of the embryo except at 45 DAF. In the embryo at 45 DAF, the cell wall boundary was more distinct. Embryos were more active and accumulated more numerous oil bodies, and the size of the oil bodies was much larger, thus the formation of oil bodies preceded their formation in the endosperm. The images of the embryo also indicate that the cell walls became thicker with the progression of seed development.

UFA content during seed development.

Oleic acid, linoleic acid, and α-linolenic acid are the predominant UFAs present in P. ostii seeds. The accumulation of these three components showed the same trends (Fig. 5). The UFAs were almost absent before 65 DAF (content at 45 and 65 DAF, <1.0%), then increased in an almost linear manner from 65 to 105 DAF. At an advanced stage of maturation (after 105 DAF), oleic acid and α-linolenic acid content plateaued, whereas linoleic acid content displayed an upward trend, slowly increasing by 3.7% during the last stage. The final contents of oleic acid, linoleic acid, and α-linolenic acid were 23.2%, 32.4%, and 38.9%, respectively.

Fig. 5.
Fig. 5.

Unsaturated fatty acid contents in Paeonia ostii seeds during follicle maturation.

Citation: HortScience horts 55, 8; 10.21273/HORTSCI15074-20

Discussion

Accumulation of oil in P. ostii seeds.

Extensive research on oil accumulation during seed development has been undertaken in a number of angiosperm species, including Pongamia pinnata (Pavithra et al., 2012) and Xanthoceras sorbifolium seeds (Zhao, 2010). All studies showed that the oil content increased gradually, whereas the water content decreased. However, in some species, such as sunflowers, arabidopsis, rapeseed, and sesame, the rapid accumulation of oil occurred at an advanced stage of seed development (Wiberg and Stymne, 1997). Oil accumulation in P. ostii seeds differed in that the rapid accumulation of oil was observed at earlier stages of development (before 85 DAF).

UFAs in P. ostii seeds.

UFA components differ among oil crops, and their accumulation patterns also differ significantly. During stages of Pongamia pinnata seed maturation, Pavithra et al. (2012) observed a significant increase in oleic acid content, whereas linoleic acid content decreased from 30.14% to 18.85% and the α-linolenic acid content remained constant, which differed from the pattern observed in P. ostii seeds. In our study, accumulation of the three predominant UFAs in P. ostii seeds showed a “slow–fast–slow” accumulation pattern, which was identical to that for oleic acid in Pongamia pinnata seeds. The dynamic changes in the three UFAs indicates it is necessary to enhance fertilizer management for P. ostii shrubs 65 to 105 DAF.

In our study, the oil content attained 23.1% at 65 DAF, but the content of the three UFAs in P. ostii seeds increased only slightly by less than 1%. The reason may be that the accumulation of fatty acids was affected by a number of fatty acid synthetases (Fofana et al., 2006; Haralampidis et al., 1998) and the synthetases were not fully activated before 65 DAF; thus, the accumulation pathway of the three major UFAs was blocked. However, the precise reason warrants additional research.

TEM observations revealed the oil bodies present in the endosperm and embryo of P. ostii seeds were similar to those of the majority of plant species. At 65 DAF, the number of oil bodies increased substantially and the oil bodies were scattered throughout the entire cell, which was very different from the images at 45 DAF. Whether the formation of the oil bodies is correlated with the rapid increase in contents of the three predominant UFAs requires further study.

Applications of MRI in seed science.

Based on MRI, two significant seed development stages were captured. At 45 to 85 DAF, P. ostii seeds were in the morphological construction stage, during which biomass and oil content increased rapidly. During this stage, although the water content decreased sharply, the plants should be irrigated timeously to ensure adequate nutritional supply for seed growth. In addition, at this stage cell enlargement and expansion are active, so fertilization should be strengthened to meet the nutrient demands of the cells. Fazli et al. (2005) observed that fertilization can affect seed yield and oil content. However, no relevant research has been conducted on P. ostii previously. Therefore, the optimal amount and type of fertilization for P. ostii needs to be explored. At 85 to 115 DAF, P. ostii seeds entered the nutrient accumulation stage. Through accumulation of nutrients at earlier stages, the carbohydrates accumulated in the seeds were converted into insoluble polymers (such as lipids) for storage, and a portion of the carbohydrates were gradually converted to UFAs.

The magnetic resonance images showed that at 45 DAF, the proton density in the seed periphery near the seedcoat was greater than that in other areas of the seed. This may indicate that development of the internal components of the seeds was not complete, so the seed was filled circumferentially from the seedcoat to the central area of the endosperm. As the seeds developed, the magnetic resonance images showed that brightness was greater at 65 DAF (Fig. 3D) than at 45 DAF (Fig. 3B). The reason may be that, during the two stages, the water content decreased significantly, yet oil began to accumulate rapidly, and the hydrogen protons lost in water were fewer than the accumulated hydrogen protons in the oil. When the seeds entered the nutrient accumulation stage [see the pseudocolor image at 105 DAF (Fig. 3J)], it was observed that the majority of the red areas in the follicles disappeared and the more yellow areas were present. The reason for the change may be that the water content of seeds in P. ostii follicles decreased, and the degrees of proton freedom decreased (i.e., part of the free water was converted to bound water).

Conclusions

Based on the magnetic resonance images, the development of P. ostii seeds can be divided into two developmental stages: a morphological construction stage (45–85 DAF) and a nutrient accumulation stage (95–115 DAF). At the early stage of seed development (45 DAF), in the endosperm, a few oil bodies were scattered at the periphery of the cell wall, and the formation of oil bodies in the endosperm was delayed compared with that in the embryo. With progression in seed development, the number and size of oil bodies in the embryo and endosperm continued to grow. The oil content in P. ostii seeds continued to accumulate during the development process and peaked at 85 DAF, whereas the water content showed the opposite trend. Also at 85 DAF, the three predominant UFAs in P. ostii seeds showed a slow–fast–slow accumulation pattern, and all accumulated rapidly from 65 to 105 DAF, which represents a critical period for cultivation of P. ostii shrubs with a high α-linolenic acid content.

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  • Fig. 1.

    Fresh and dry weight of Paeonia ostii seeds during follicle maturation. The different lowercase letters are significantly different according Duncan’s multiple range test at P ≤ 0.05.

  • Fig. 2.

    Water and oil contents of Paeonia ostii seeds during follicle maturation. The different lowercase letters are significantly different according Duncan’s multiple range test at P ≤ 0.05.

  • Fig. 3.

    Magnetic resonance images of Paeonia ostii follicles during maturation. Grayscale and pseudo-color images of follicles (A, B) 45 d after flowering (DAF), (C, D) 65 DAF, (E, F) 85 DAF, (G, H) 95 DAF, (I, J) 105 DAF, and (K, L) 115 DAF. The color scale in the legend represents, from deep red to yellow to deep blue, a gradual decrease in proton density.

  • Fig. 4.

    Ultrastructure of endosperm and embryo cells of Paeonia ostii seeds at 45, 65, 85, and 115 d after flowering (DAF) visualized by transmission electron microscopy. OB, oil body; BG, highly electron-dense substance; CW, cell wall; N, nucleus.

  • Fig. 5.

    Unsaturated fatty acid contents in Paeonia ostii seeds during follicle maturation.

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Yuting Zou Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Yanan Wang Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Mingwei Zhu Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Shuxian Li Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forest Science, Nanjing Forestry University, Nanjing 210037, China

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Qiuyue Ma Institute of Leisure Agriculture in Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

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Contributor Notes

We acknowledge funding received from the Province Graduate Research and Practice Innovation of Jiangsu for the project “Study on the mechanism of oil accumulation during the development of Paeonia ostii seeds” (KYCX18-0961).

We thank Robert McKenzie, from Liwen Bianji, Edanz Group China, for editing the English of a draft of this manuscript.

S.L. is the corresponding author. E-mail: shuxianli@njfu.com.cn.

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