Abstract
The kernel of the macadamia nut (Macadamia integrifolia and M. tetraphylla) is very high in oil, accounting for about three -quarters of their mass. In the current investigation, oil extracts from 20 breeding accessions and 14 cultivars had a range of 12.3% to 17.0% saturated fat, averaging 14.2%. Although all samples were found to be very high in “healthy” monounsaturated fats, the level of saturated fat slightly exceeds that of many other nuts that are able to make qualified health claims. The lowest saturated fat content (12.3%) corresponded to 4.6 g saturated fat/50 g kernels, which was slightly greater than the 4.0 g maximum. Despite this, potential exists to develop a reduced-saturated fat macadamia by combining characteristics found in different lines. The current trial indicates that lower total saturated fat was associated with a stronger ability to partition C16 and C18 fats to their monounsaturated fatty acids, or to elongate C16:0 to C18:0 and subsequently desaturate C18:0 to C18:1. It was also observed that the pollinizer parent is likely to have an influence on saturated fat content, although this would need to be confirmed in controlled pollination trials. Macadamia varieties generally outcross, and because the edible kernel (embryo) is formed from a pollinated ovule, it is likely any future reduced-saturated fat line would also require a reduced-saturated fat pollinizer parent.
Macadamia nut kernels (Macadamia integrifolia, M. tetraphylla) are very high in oil, accounting for about three-quarters of their weight (Saleeb et al., 1973). The oil itself consists of ≈77% to 80% monounsaturated fat, 1% to 7% polyunsaturated fat, and the remaining 14% to 21% saturated fat (Aquino-Bolaños et al., 2017; Beuchat and Worthington, 1978; Carrillo et al., 2017; Cavaletto et al., 1966; Saleeb et al., 1973). Although unsaturated fats are considered “good” for cardiovascular health, there is a general consensus that saturated fats are detrimental (Clifton and Keogh, 2017; Sacks et al., 2017; Wang and Hu, 2017). And although the majority of fats in macadamia are “good,” the level of saturated fat present (>8 g/100 g kernels) does restrict label health claims both in Australia (Front-of-Pack Labelling Secretariat, 2018) and in the United States (Food and Drug Administration, 2003, 2017). Fatty acid profiles vary widely between nut species (Ros and Mataix, 2006) and may range widely within a species (Aquino-Bolaños et al., 2017; Mereles et al., 2017). Variation of fatty acid profiles is largely genetically based (Rodríquez et al., 2015), and is influenced primarily by the presence and activity of enzymes regulating fatty acid synthesis, elongation, and desaturation during biosynthesis (Barker et al., 2007; Brown et al., 2009). The endpoint of synthesis is usually the saturated fatty acids palmitate (C16:0) and stearate (C18:0), with the latter predominating (Harwood, 2018). Once produced, they can be subject to further elongation and desaturation. Examples of desaturation in macadamia include palmitic acid (C16:0) to palmitoleic acid (C16:1), and stearic acid (C18:0) to oleic acid (C18:1), whereas an example of elongation includes palmitic acid (C16:0) to stearic acid (C18:0).
The following investigation explores the variability in fatty acid profiles of a range of commercial cultivars and accessions in the Australian macadamia nut breeding program. The aim of this analysis was to identify the existence of reduced-saturated fat varieties, or accessions that may have potential as prospective parents for breeding a low- or a reduced-saturated fat variety. Because saturated fatty acids are the precursors of mono- and polyunsaturated fatty acids, accessions with greater unsaturated-to-saturated fatty acid ratios may further serve as a useful starting point for producing a variety with both reduced saturated fat and greater unsaturated fat.
Materials and Methods
Fourteen cultivars and 20 breeding accessions from the Australian macadamia breeding program were harvested at commercial maturity from the Tiaro (Queensland) germplasm collection in 2016. The pollination parent was uncontrolled and, when possible, nuts were harvested from two different trees of each line, spaced as distant as possible from each other within the germplasm collection. All trees were grown under the same fertilizer and irrigation regimes on a uniform soil type.
The nuts were dehusked and dried in an incubator (35 °C for 2 d, 45 °C for 2 d, 55 °C for 2 d) to ≈1% to 2% moisture content. Nuts were hand-cracked, and five kernels were selected randomly for oil extraction. Oil was extracted with a hydraulic press operated at 24 °C. Approximately 3 mL oil was collected as a composite sample. Approximately 0.1 g oil was dissolved in 1.6 mL hexane before adding 100 µL 2 M methanolic KOH. The mixture was shaken for 30 s and then centrifuged at 960 gn for 2 min. The hexane layer was collected and filtered for gas chromatography–mass spectrometry analysis. One microliter of the filtered hexane layer was injected into a Shimadzu GC-MS system (Shimadzu, Kyoto, Japan), consisting of a gas chromatograph (Shimadzu GC-2010 Plus) coupled with a mass spectrometer (Shimadzu MS-TQ8040) using a Shimadzu AOC 6000 autosampler. The gas chromatograph was fitted with an Rtx-5MS column (length, 30 m; thickness, 0.25 μm; diameter, 0.25 mm) (Restek, Bellefonte, PA). The injector was held at 240 °C. The column oven was held at 80 °C for 1 min, then increased to 150 °C at a rate of 30 °C/min. The column temperature was then increased to 180 °C at a rate of 4 °C/min, and then increased to 280 °C at a rate of 3 °C/min. Column flow rate was 1.34 mL/min, total flow rate was 193.9 mL/min, and the split ratio was 140.0. The carrier gas was nitrogen (high purity) and the column pressure 91.3 kPa. Fatty acid methyl esters were identified by comparison of retention time, molecular mass, and fragment ions. Oil concentration was determined as a percentage of total oil based on the ratio of individual peak area to total peak area, using data processing software (Labsolutions Insight software, Version 3.2, Shimadzu Oceania, Sydney, Australia).
An estimate of C16 and C18 fatty acid desaturation and elongation partitioning ratios were calculated based on the ratios of different fatty acid groups found in the fatty acid profile. For example, the monounsaturated fatty acid C16:1 is formed (desaturated) from its saturated precursor C16:0. But, C16:0 can also be elongated to C18:0, which can be further elongated to C20:0, or desaturated to C18:1. Therefore, the ratio of C16:1 to its precursor C16:0, plus these other downstream fatty acids (C18:0, C20:0, C18:1, etc.), provides an indication of how well a particular accession partitions fatty acids toward C16:1. Similarly, an estimate can be made as to how well an accession partitions C18:0 toward C18:1, and how well C16:0 is elongated to C18:0. These calculations assume that plant fatty acids are formed principally through elongation and desaturation, rather than shortening or saturation. Regular fatty acid desaturation of C16:0 to C16:1 and C18:0 to C18:1, and elongation of C16:0 to C18:0 was assumed, based on the general greater higher plant fatty acid biosynthesis pathway (Barker et al., 2007). It was also assumed that C20:1 and C22:1 were formed from the elongation of C18:1 and C20:1, respectively, rather than direct desaturation of C20:0 and C22:0. Desaturation and elongation partitioning ratios were subsequently based on the ratios of the following fatty acids:
C16 desaturation: C16:1/(C16:0 + C18:0 + C18:1 + C18:2 + C18:3 + C20:0 + C20:1 + C22:0 + C22:1)
C18 desaturation: (C18:1 + C18:2 + C18:3 + C20:1 + C22:1)/(C18:0 + C20:0 + C22:0)
C16 elongation: (C18:0 + C18:1 + C18:2 + C18:3 + C20:0 + C20:1 + C22:0 + C22:1)/(C16:0 + C16:1)
Multiple linear regression analysis between total saturated fat content (measured as a percentage) and desaturation and elongation partitioning ratios was conducted using Genstat statistical software (Version 18, VSN International, Hemel Hempstead, UK). Differences in fatty acid content between accessions were not compared statistically because of the low tree number within the orchard germplasm collection. Fatty acid data from a composite sample from each of two trees per accession were collected to generate partitioning ratios as well as an indication of potential intertree variation within a mixed orchard.
Results
The major fatty acids in all cultivars and breeding accessions were the monounsaturated fats: oleic acid (C18:1) and palmitoleic acid (C16:1). The concentration of oleic and palmitic acid ranged from 57% to 67% and 14% to 24%, respectively. The next highest concentrated fatty acids were the saturated fats: palmitic acid (C16:0, 7% to 10%), stearic acid (C18:0, 2% to 4%), and arachidic acid (C20:0, 2%). The remaining unsaturated fatty acids (C18:2, C18:3, C20:1, and C22:1) were each generally less than 2% (Tables 1 and 2). In addition to the major saturated fatty acids, other saturated fats detected in macadamia included, in order of concentration, myristic acid (C14:0), behenic acid (C22:0), and lauric acid (C12:0).
Kernel oil fatty acid profiles and desaturation/elongation partitioning ratios of cultivars.


Kernel oil fatty acid profiles and desaturation/elongation partitioning ratios of breeding accessions. The number following the cultivar or accession name is the tree number.


Total saturated fatty acid concentration across the lines varied over a narrow range of 12.3% to 17.0% (Fig. 1), with an average of 14.2%. The greatest average saturated fat percentage was observed in ‘HAES246’, whereas the least was observed in ‘A376’. Different tree location of the same accession was also observed to have an impact on saturated fat concentration. Although in some cultivars and accessions (‘A447’, ‘A403’, Q, ‘A422’) intertree variability was quite small at, for example, 0.1%, in others (S, ‘A16’) it was as high as 4.1%.

Variation in total saturated fat content between two trees of the same cultivar/accession in different locations within the orchard. The midline (dashed) indicates the average of the two trees (upper and lower lines). Cultivars or accessions with only one tree are not shown.
Citation: HortScience horts 54, 4; 10.21273/HORTSCI13418-18

Variation in total saturated fat content between two trees of the same cultivar/accession in different locations within the orchard. The midline (dashed) indicates the average of the two trees (upper and lower lines). Cultivars or accessions with only one tree are not shown.
Citation: HortScience horts 54, 4; 10.21273/HORTSCI13418-18
Variation in total saturated fat content between two trees of the same cultivar/accession in different locations within the orchard. The midline (dashed) indicates the average of the two trees (upper and lower lines). Cultivars or accessions with only one tree are not shown.
Citation: HortScience horts 54, 4; 10.21273/HORTSCI13418-18
As a simple comparison of desaturation efficiency, the partitioning ratio of unsaturated to saturated fat varied between fatty acids of different chain length. Across all accessions, the ratio of unsaturated palmitoleic acid (C16:1) to saturated palmitic acid (C16:0) was much less than the ratio of C18 unsaturated (18:1, 18:2, C18:3) to saturated (18:0) fats (Tables 1 and 2). Consequently, palmitic acid (C16:0) was consistently the highest saturated fat present in all accessions. Desaturation of stearic acid (C18:0) to oleic acid (C18:1) appeared to be much more efficient.
Total saturated fat was found to be correlated to desaturation and elongation partitioning ratios. C18-desaturation partitioning, rather than C16-desaturation partitioning, was observed to account for a large percentage of variance (r2 = 52%) in total saturated fatty acids (Table 3). However, although C16-desaturation and C16-elongation partitioning accounted for less than 2% variance individually, combining C18-desaturation, C16-desaturation, and C16-elongation partitioning was able to explain 88% of variance of total saturated fat concentration (Table 3). Interestingly, C16-elongation partitioning correlated highly and negatively (r2 = 92%) with C16-desaturation partitioning (Fig. 2A). Consequently, the lowest total saturated fat values tended to be achieved with a combination of high C18-desaturation and high C16-elongation partitioning, or high C18-desaturation with high C16-desatuation partitioning, but not both. C18-desaturation partitioning was also found to have no correlation with the C16-desaturation partitioning ratio (Fig. 2B).
Linear regression relationships between total saturated fat (%) and fatty acid desaturation and elongation partitioning ratios.



Correlation relationships between (A) C16-desaturase and C16-elongase, and (B) C18-desaturase partitioning ratios.
Citation: HortScience horts 54, 4; 10.21273/HORTSCI13418-18

Correlation relationships between (A) C16-desaturase and C16-elongase, and (B) C18-desaturase partitioning ratios.
Citation: HortScience horts 54, 4; 10.21273/HORTSCI13418-18
Correlation relationships between (A) C16-desaturase and C16-elongase, and (B) C18-desaturase partitioning ratios.
Citation: HortScience horts 54, 4; 10.21273/HORTSCI13418-18
Discussion
The fatty acid profiles observed in the accessions and cultivars assessed in the current trial indicated that total saturated fat varied within a narrow range of 12.3% to 17.0%. The average was 14.2%, which is similar to that published for macadamia oil in the Food Standards Australia and New Zealand (2017) nutrition tables. Because the lowest saturated fat concentration (i.e., 12.3%) is only 13% less than the Food Standards Australia and New Zealand reference value of 14.2%, this oil is insufficient to qualify as a “reduced” saturated fat product, which requires at least a 25% reduction relative to the reference value (Federal Register of Legislation, 2017). The current saturated fat content also slightly reduces the Australian “health star rating” relative to many other nuts (Front-of-Pack Labelling Secretariat, 2018). Similarly, on a whole-kernel value, which consists of 75% oil, the amount of saturated fat (4.6 g/50 g kernel) exceeds that required (<4.0 g/50g) by the U.S. Food and Drug Administration for an unqualified nut health claim possible for many other commercial nuts (e.g., almond, pistachio, peanut) (Food and Drug Administration, 2003). Clearly, assessment of other macadamia accessions, including those outside the M. integrifolia and M. tetraphylla species, or targeted breeding, is necessary to reach lower total saturated fat concentrations.
In agreement with previous reports (Aquino-Bolaños et al., 2017; Beuchat and Worthington, 1978; Carrillo et al., 2017; Cavaletto et al., 1966; Saleeb et al., 1973), palmitic (C16:0) and stearic acid (C18:0) were the major saturated fatty acids present in the breeding accessions/cultivars assessed. Palmitic acid always constituted the highest fatty acid, with about three times as much as stearic acid. From the data (Tables 1 and 2), it would appear that endogenous desaturation of palmitic to palmitoleic acid (C16:1) is considerably less efficient than stearic to oleic (C18:1). It was also apparent that desaturation partitioning of C16:0 was not at all correlated to desaturation partitioning of C18:0 (Fig. 2B), which would support the suggestion by Gummeson et al. (2000) that the enzyme responsible for C18 desaturation (Δ9-stearoyl-acyl-carrier-protein) is unlikely to be the enzyme responsible for C16 desaturation.
Total saturated fat content was found to correlate significantly with the C18-desaturase partitioning ratio (r2 = 52%), but correlated poorly with either C16-desaturase or C16-elongase partitioning ratios (Table 3). This is probably the result of the generally greater conversion of C18:0 to C18:1, and the fact that the denominator of this equation included only the desaturated fats (C18:0, C20:0, and C22:0), as C20:1 and C22:1 are thought to be formed via elongation of C18:1 and C20:1, respectively, rather than by direct desaturation of C20:0 and C22:0 (Barker et al., 2007).
Interestingly, addition of C16-desaturase and C16-elongation partitioning ratios to the correlation equation further accounted for the variation observed (r2 = 88%) in saturated fat content (Table 3). It would appear that accessions or cultivars with the lowest saturated fat content had 1) a combination of high C18-desaturation partitioning and high C16-desaturation partitioning or 2) a combination of high C18-desaturation partitioning and high C16-elongation partitioning. Because C16-desaturation and C16-elongation partitioning ratios correlate strongly and negatively (Fig. 2A), it was impossible to have both in the same accession. This indicates that C16 desaturase and C16 elongase are competing for the same substrate (C16:0). Putting all three factors in the same equation accounted for both scenarios leading to lower total saturated fat.
Different saturated fatty acid profiles for trees of the same accession positioned at different locations within the orchard were also observed in the current trial (Fig. 1). It is possible that these differences were the result of different pollinizers for each tree. Macadamia has a strong tendency to outcross (Sedgley et al., 1990), and the kernel tissue is made up of genetic material from both the maternal and paternal genome. It is therefore likely that the pollinizer would modify the fatty acid profile of the maternal accession, although a controlled pollination trial would be necessary to confirm this. Such a situation should be taken into account when developing a reduced-saturated fat or increased-unsaturated fat breeding line. In the current study, the pollinizers were not identified, although it is likely they may have been the adjacent flowering trees.
The current study indicates that saturated fat content in macadamia oil varies within the range of 12.3% to 17.0%. The predominant saturated fatty acids, in order of concentration, were palmitic, stearic, and behenic acid. In general, desaturation of palmitic acid appeared less efficient than desaturation of stearic acid. Although the majority of lines with less saturated fat content had a combination of both higher C16- and higher C18-desaturation partitioning ratios, some lines had a combination of higher C18-desaturation and higher C16-elongation partitioning ratios. Both combinations were able to achieve a lower saturated fat content as a result of the latter shifting the fatty acid flux toward C18:0, which appears to be desaturated more efficiently. Interestingly, the kernel fatty acid profile is likely to be affected by the pollinizer, which should be considered in future assessments. Development of a reduced-saturated fat macadamia will likely require both low-saturated fat maternal and paternal parents.
Literature Cited
Aquino-Bolaños, E.N., Mapel-Velazco, L., Martín-del-Campo, S.T., Chávez-Servia, J.L., Martínez, A.J. & Verdalet-Guzmán, I. 2017 Fatty acids profile of oil from nine varieties of macadamia nut Intl. J. Food Prop. 20 1262 1269
Barker, G.C., Larson, T.R., Graham, I.A., Lynn, J.R. & King, G.J. 2007 Novel insights into seed fatty acid synthesis and modification pathways from genetic diversity and quantitative trait loci analysis of the Brassica C genome Plant Physiol. 144 1827 1842
Beuchat, L. & Worthington, R.E. 1978 Fatty acid composition of tree nut oils J. Food Technol. 13 355 358
Brown, A.P., Slabas, A.R. & Rafferty, J.B. 2009 Fatty acid biosynthesis in plants: Metabolic pathways, structure, and organization, p. 11–34. In: H. Wada and N. Murata (eds.). Lipids in Photosynthesis. Springer, Dordrecht, Netherlands
Carrillo, W., Carpio, C., Morales, D., Vilcacundo, E. & Alvarez, M. 2017 Fatty acids composition in macadamia seed oil (Macadamia integrifolia) from Ecuador Asian J. Pharm. Clin. Res. 10 303 306
Cavaletto, C.G., De la Cruz, A., Ross, E. & Yamamoto, H.Y. 1966 Factors affecting macadamia nut stability: 1. Raw kernels Food Technol. 20 1084 1087
Clifton, P.M. & Keogh, J.B. 2017 A systematic review of the effect of dietary saturated and polyunsaturated fat on heart disease Nutr. Metab. Cardiovasc. Dis. 27 1060 1080
Federal Register of Legislation 2017 Australia New Zealand Food Standards Code: Schedule 4: Nutrition, health and related claims. <https://www.legislation.gov.au/Details/F2017C00711>.
Food and Drug Administration 2003 Qualified health claims: Letter of enforcement discretion: Nuts and coronary heart disease. Docket no. 02P-0505. <http://wayback.archive-it.org/7993/20171114183724/https://www.fda.gov/Food/IngredientsPackagingLabeling/LabelingNutrition/ucm072926.htm>.
Food and Drug Administration 2017 Petition for a qualified health claim for macadamia nuts and reduced risk of coronary heart disease. Docket no. FDA-2015-Q-4850. <https://www.fda.gov/downloads/Food/LabelingNutrition/UCM568057.pdf>.
Food Standards Australia New Zealand 2017 NUTTAB 2010 online searchable database: Oil, macadamia. Food ID: 04C10086. <http://www.foodstandards.gov.au/science/monitoringnutrients/nutrientables/nuttab/Pages/default.aspx>.
Front-of-Pack Labelling Secretariat 2018 Guide for industry to the Health Star Rating Calculator, version 6. <http://healthstarrating.gov.au/internet/healthstarrating/publishing.nsf/Content/E380CCCA07E1E42FCA257DA500196044/$File/Guide%20for%20Industry%20to%20the%20Health%20Star%20Rating%20Calculator.pdf>.
Gummeson, P.O., Lenman, M., Lee, M., Singh, S. & Stymneet, S. 2000 Characterisation of acyl-ACP desaturases from Macadamia integrifolia Maiden & Betche and Nerium oleander L Plant Sci. 154 53 60
Harwood, J.L. 2018 Plant fatty acid biosynthesis. AOCS Lipid Library. <http://lipidlibrary.aocs.org/Biochemistry/content.cfm?ItemNumber=40304>.
Mereles, L.G., Ferro, E.A., Alvarenga, N.L., Caballero, S.B., Wiszovaty, L.N., Piris, P.A. & Michajluk, B.J. 2017 Chemical composition of Macadamia integrifolia (Maiden and Betche) nuts from Paraguay Intl. Food Res. J. 24 2599 2608
Rodríguez, M.F.R., Sánchez-García, A., Salas, J.J., Garces, R. & Martínez-Force, E. 2015 Characterization of soluble acyl-ACP desaturases from Camelina sativa, Macadamia tetraphylla and Dolichandra unguis-cati J. Plant Physiol. 178 35 42
Ros, E. & Mataix, J. 2006 Fatty acid composition of nuts: Implications for cardiovascular health Brit. J. Nutr. 96 Suppl. 2 S29 S35
Sacks, F.M., Lichtenstein, A.H., Wu, J.H.Y., Appel, L.J., Creager, M.A., Kris-Etherton, P.M., Miller, M., Rimm, E.B., Rudel, L.L., Robinson, J.G., Stone, N.J. & Van Horn, L.V. 2017 Dietary fats and cardiovascular disease: A presidential advisory from the American Heart Association Circulation 136 e1 e23
Saleeb, W.F., Yermanos, D.M., Huszar, C.K., Storey, W.B. & Labanauskas, C.K. 1973 The oil and protein in nuts of Macadamia tetraphylla L. Johnson, Macadamia integrifolia Maiden and Betche, and their F1 hybrid J. Amer. Soc. Hort. Sci. 98 453 456
Sedgley, M., Bell, F.D.H., Bell, D., Winks, C.W. & Pattison, S.J. 1990 Self- and cross-compatibility of macadamia cultivars J. Hort. Sci. 65 205 213
Wang, D.D. & Hu, F.B. 2017 Dietary fat and risk of cardiovascular disease: Recent controversies and advances Annu. Rev. Nutr. 37 423 446