Characterization of Partial Resistance to Black Spot Disease of Rosa sp.

in HortScience

Black spot disease, caused by the fungus Diplocarpon rosae Wolf, is one of the most serious diseases of garden roses. Both complete (vertical) resistance conditioned by dominant Rdr genes and partial (horizontal) resistance conditioned by multiple genes have been described. The use of resistant rose cultivars would reduce the demand of agrochemical applications. The characterization of 16 genotypes for resistance to black spot using two laboratory assays, the detached leaf assay (DLA) and the whole plant inoculation (WPI) approach, indicated that these techniques were well correlated. Thus, either method could be used to assess the resistance of the plants to black spot. Fifteen diploid hybrid populations from 10 parents segregating for partial (horizontal) resistance to black spot derived from Rosa wichuraiana ‘Basye’s Thornless’ (RW) were assessed for black spot resistance by quantifying the percentage of the leaf area with symptoms (LAS) and lesion length (LL) measured by the diameter of the largest lesion per leaf in DLAs. The narrow-sense heritability of partial resistance to black spot as measured by LAS and LL data of DLA was estimated to be from 0.28 to 0.43 when calculated with a genetic variance analysis and from 0.74 to 0.86 when generated from offspring–midparent regression. This suggests that the development of rose cultivars with high levels of stable partial resistance to black spot is a feasible approach for the rose industry.

Contributor Notes

This work was partially funded by Monsanto Fellows in Plant Breeding program, the Robert E. Basye Endowment in Rose Genetics, and the USDA’s National Institute of Food and Agriculture (NIFA) Specialty Crop Research Initiative project, “RosBREED: Combining disease resistance with horticultural quality in new Rosaceous cultivars.”

We thank the Hokanson lab at the University of Minnesota for supplying race 8 of Diplocarpon rosae used in this work.

Corresponding author. E-mail: dbyrne@tamu.edu.

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Figures

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    (A) Spores bearing acervuli on infected leaf surface of ‘Cl. Pinkie’. (B) Diagrammatic representation of leaf area with symptoms of black spot disease at 1%, 5%, 10%, 25%, 50%, or 75% in detached leaf assay.

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    Correlation of leaf area with symptoms (LAS) and lesion length (LL) measurements of partial resistance after infection with race 8 of Diplocarpon rosae with the detached leaf assay method.

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    Correlation of the partial resistance to black spot race 8 as measured by leaf area with symptoms (LAS) and lesion length (LL) in detached leaf assays among individual seedlings of 15 diploid rose progenies.

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    The slope of offspring–midparent regressions estimates the narrow-sense heritability of 15 diploid populations measured by (A) leaf area with symptoms (LAS) and (B) lesion length (LL) from detached leaf assay inoculated by race 8 of Diplocarpon rosae with R2 indicating the fitness of the regression. Original data were transformed using a square root.

References

AmericanHort2014Rose rosette disease targeted in 2014. 28 Apr. 2014. <http://americanhort.theknowledgecenter.com/AmericanHortNews/index.cfm?view=detail&colid=147&cid=421&mid=6001>.

BlechertO.DebenerT.2005Morphological characterization of the interaction between Diplocarpon rosae and various rose speciesPlant Pathol.548290

ByrneD.H.2015Advances in rose breeding and genetics in North AmericaActa Hort.10648998

ByrneD.H.AndersonN.OrwatM.SoulesV.2010Field assessment of black spot resistance in roses in a hot humid climateActa Hort.870115120

Carlson-NilssonB.U.2002Variation in Rosa with emphasis on the improvement of winter hardiness and resistance to Marssonina rosae (Black spot). Swedish Univ. of Agr. Sci. Uppsala PhD Diss

ConnorA.M.StephensM.J.HallH.K.AlspachP.A.2005Variation and heritabilities of antioxidant activity and total phenolic content estimated from a red raspberry factorial experimentJ. Amer. Soc. Hort. Sci.130403411

DebenerT.ByrneD.2014Disease resistance breeding in rose: Current status and potential of biotechnological toolsPlant Sci.228107117

DietersM.J.WhiteT.L.LittellR.C.HodgeG.R.1995Application of approximate variances of variance components and their ratios in genetic testsTheor. Appl. Genet.911524

FalconerD.S.MackayT.F.C.1996Introduction to quantitative genetics. 4th ed. Prentice Hall New York NY

GachomoE.W.DehneH.W.SteinerU.2006Microscopic evidence for the hemibiotrophic nature of Diplocarpon rosae, cause of black spot disease of rosePhysiol. Mol. Plant Pathol.698692

GudinS.2000Rose: Genetics and breedingPlant Breeding Rev.17159189

HallauerA.R.CarenaM.J.Miranda FilhoJ.B.2010Quantitative genetics in maize breeding. Springer New York NY

HattendorfA.LindeM.MattieschL.DebenerT.KaufmannH.2004Genetic analysis of rose resistance genes and their localisation in the rose genomeActa Hort.651123130

HollandJ.B.NyquistW.E.Crevantes-MartinezC.T.2003Estimating and interpreting heritability for plant breeding: An updatePlant Breeding Rev.229112

HorstR.K.CloydR.A.2007Compendium of rose diseases and pests. 2nd ed. Amer. Phytopathol. Soc. Saint Paul MN

HuttonS.2012The future of the rose industryAmer. Rose413637

JohanssonM.Kronestedt-RobardsE.RobardsA.1992Rose leaf structure in relation to different stages of micropropagationProtoplasma166165176

KrüssmannG.1981The complete book of roses. Timber Press Portland OR

LittellR.C.MillikenG.A.StroupW.W.WolfingerR.D.1996SAS system for mixed models. SAS Institute Inc. Cary NC

MarriottM.2003History of roses in cultivation/modern (Post-1800) p. 402–409. In: A.V. Roberts T. Debener and S. Gudin (eds.). Encyclopedia of rose science. Elsevier Oxford UK

NautaM.M.SpoonerB.M.2000British Dermateaceae: 4B. Dermateoideae gerna B-EMycologist142128

NoackR.2003Breeding/selection strategies for disease and pest resistance p. 49–55. In: A.V. Roberts T. Debener and S. Gudin (eds.). Encyclopedia of rose science. Elsevier Oxford UK

PalmerJ.G.SeminukP.StewartR.N.1966Roses and blackspot. II. Seasonal variation in host susceptibility and decline of virulence in culture of conidia from Diplocarpon rosaePhytopathology5612831286

ParlevlietJ.E.1981Race-non-specific disease resistance p. 47–54. In: J.F. Jenkyn and R.T. Plumb (eds.). Strategies for the control of cereal disease. Blackwell Oxford UK

RazaliN.M.WahY.B.2011Power comparisons of Shapiro-Wilk, Kolmogorov-Smirnov, Lilliefors and Anderson-Darling testsJ. Stat. Modeling Analytics22133

ShepherdR.E.1954History of the rose. Macmillan New York NY

ShupertD.2005The inheritance of several traits in three diploid interspecific rose populations. Texas A&M Univ. College Station MS Thesis

von MalekB.DebenerT.1998Genetic analysis of resistance to blackspot (Diplocarpon rosae) in tetraploid rosesTheor. Appl. Genet.96228231

WaliczekT.M.ByrneD.H.HolemanD.2015Growers’ and consumers’ knowledge, attitudes and opinions regarding roses available for purchaseActa Hort.1064235240

WhitakerV.M.HokansonS.C.2009aPartial resistance to black spot disease in diploid and tetraploid roses: General combining ability and implications for breeding and selectionEuphytica169421429

WhitakerV.M.HokansonS.C.2009bBreeding roses for disease resistance p. 277–324. In: J. Janick (ed.). Plant breeding reviews. Vol. 31. Wiley New York NY

WhitakerV.M.BradeenJ.M.DebenerT.BiberA.HokansonS.C.2010aRdr3, a novel locus conferring black spot disease resistance in tetraploid rose: Genetic analysis, LRR profiling, and SCAR marker developmentTheor. Appl. Genet.120573585

WhitakerV.M.DebenerT.RobertsA.V.HokansonS.C.2010bA standard set of host differentials and unified nomenclature for an international collection of Diplocarpon rosae racesPlant Pathol.59745752

XueA.G.DavidsonC.G.1998Components of partial resistance to black spot disease (Diplocarpon rosae Wolf) in garden rosesHortScience339699

YokoyaK.KandasamyK.WalkerS.MandegaranZ.RobertsA.B.2000Resistance of roses to pathotypes of Diplocarpon rosaeAnn. Appl. Biol.1361520

ZlesakD.C.WhitakerV.M.GeorgeS.HokansonS.C.2010Evaluation of roses from the Earth-Kind® Trials: Black spot (Diplocarpon rosae Wolf) resistance and ploidyHortScience4517791787

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