Susceptibility of stone fruits to chilling injury (CI) depends on genotype, maturity, storage temperature, length of storage, and preharvest and postharvest manipulation (Lurie and Crisosto, 2005). CI symptoms include lack of juiciness (flesh mealiness or woolliness), FB, black pit cavity, flesh translucency (gel breakdown), red pigment accumulation (bleeding), lack of flavor, and failure to ripen (Lurie and Crisosto, 2005). Nectarine FB is a genetic disorder that can be triggered by a combination of factors such as cultural practices (soil fertilization, irrigation management), postharvest temperature, and storage and shipping processes (Crisosto et al., 1999; Mitchell, 1987). Mineral nutrition is critical for tree fruit growth and production (Johnson and Uriu, 1989), but little is known about the role of nutrition in cold storage disorders, CI expression in fresh cut fruit, and fruit consumption by consumers. Fruit nutrition research has mainly focused on optimizing tree growth and yield with limited attention paid to its effect on stone fruit quality (Crisosto et al., 1997). Studies on apples (Bramlage et al., 1980; Crisosto et al., 1997) and plums (Kotze et al., 1987) reported an influence of nutrient deficiencies on internal breakdown. Therefore, further research on the influence of preharvest manipulations such as mineral nutrition, irrigation, and cropload on stone fruit postharvest quality and cold storage performance is needed.
Fruit tissue enzymatic browning is a consequence of oxidative degradation of phenolic compounds by polyphenol oxidase (PPO), which leads to production of quinones that polymerize rapidly to form brown-colored products (Kader and Chordas, 1984). The concentration of phenolic compounds, the activity of PPO, and the presence of available oxygen are strongly related to enzymatic browning (Mayer and Harel, 1979). Phenolic composition and concentration are important contributors to fruit antioxidant capacity, which is highly desirable, because it provides several benefits to human health (Robards et al., 1999). In addition, phenolic compounds, in combination with other factors, can improve brown rot (Monilinia fructicola) resistance in peaches by acting directly on cutinase and preventing the penetration of this fungal infection within the fruit flesh (Bostock et al., 1999).
Flesh browning can be triggered by fruit bruising; by exposure to oxygen in fresh cut, sliced, and pulped forms; or by thawing fruit after prolonged freezing. In addition, lye-peeling of canned fruit also strongly influences the browning potential of the fruit (Vamosvigyazo, 1981). Thus, fruit handling and processing are critical factors underlying browning potential, because these activities can damage cell structure. Enzymatic FB is a major component of postharvest CI problems in stone fruit. It is also a commercial problem, because browning can affect fruit quality in fresh or canned forms. Any enzymatic browning is a major industrial concern because the altered sensory and visual characteristics are unattractive to consumers in addition to the quality loss resulting from decreased concentrations of phenolic compounds. It is highly desirable, therefore, to have fruit with high concentrations of phenolic compounds but low postharvest FB during and after cold storage (Ogundiwin et al., 2008). A high concentration of antioxidants such as ascorbic acid is also highly desirable in fruits, because it may prevent some degree of enzymatic browning by inhibiting PPO activity (Teisson, 1972). Furthermore, quinone compounds can be reduced by ascorbic acids, regenerating phenolics and inhibiting browning.
Some stone fruit species are more susceptible to FB than others. Some peach and nectarine cultivars from California breeding programs are highly susceptible to FB, reducing their storage potential (Crisosto et al., 2008, 2009). This study relates nutrient deficiencies to FB and its precursors phenolic concentration, PPO activity, and antioxidant concentration in a high FB, susceptible cultivar. We hypothesize that low concentrations of nutrients and phosphorus (P) will lead to greater FB by modifying the membrane permeability to make browning reaction substrates more readily available.
Amiot, M.J., Fleuriet, A., Cheynier, V. & Nicolas, J. 1996 Phenolic compounds and oxidative mechanisms in fruit and vegetables. Annual Proc. of the Phytochemical Society of Europe. 41:51.
A.O.A.C., I 2006 AOAC Official Method 972.43, Microchemical determination of carbon, hydrogen, and nitrogen, automated method, p. 5–6. In: International, A. (ed.), Official Methods of Analysis of AOAC International. AOAC International, Gaithersburg, MD.
Bostock, R.M., Wilcox, S.M., Wang, G. & Adaskaveg, J.E. 1999 Suppression of Monilinia fructicola cutinase production by peach fruit surface phenolic acids Physiol. Mol. Plant Pathol. 54 37 50
Bramlage, W.J., Drake, M. & Lord, W.J. 1980 The influence of mineral nutrition on the quality and storage performance of pome fruits grown in North America Acta Hort. 92 29 40
Brand-Williams, W., Cuvelier, M.E. & Berset, C. 1995 Use of a free radical method to evaluate antioxidant activity Lebensmittel-Wissenschaft and Technologie 28 25 30
Cheng, G.W.W. & Crisosto, C.H. 1995 Browning potential, phenolic composition, and polyphenoloxidase activity of buffer extracts of peach and nectarine skin tissue J. Amer. Soc. Hort. Sci. 120 835 838
Childers, N.F. & Sherman, W.B. 1988 The peach: Culture, cultivars, breeding, propagation, nutrition, training and pruning, diseases and insects, harvesting, storage and marketing. Horticultural Publications, New Brunswick, NJ.
Crisosto, C.H., Crisosto, G.M. & Day, K.R. 2008 Market life update for peach, nectarine, and plum cultivars grown in California Advances in Horticultural Science 22 201 204
Crisosto, C.H., Johnson, R.S., DeJong, T. & Day, K.R. 1997 Orchard factors affecting postharvest stone fruit quality HortScience 32 820 823
Crisosto, C.H., Johnson, R.S., Luza, J.G. & Crisosto, G.M. 1994 Irrigation regimes affect fruit soluble solids concentration and rate of water-loss of O'Henry peaches HortScience 29 1169 1171
Crisosto, C.H., Lurie, S. & Retamales, J. 2009 Stone fruits, p. 287–315. In: Yahia, E.M. (ed.). Modified and controlled atmospheres for the storage, transportation, and packaging of horticultural commodities. CRC Press/Taylor & Francis, Boca Raton, FL.
Crisosto, C.H., Mitchell, F.G. & Ju, Z.G. 1999 Susceptibility to chilling injury of peach, nectarine, and plum cultivars grown in California HortScience 34 1116 1118
Horneck, D.A. & Miller, R.O. 1998 Determination of total nitrogen in plant tissue, p. 75–83. In: Kalra, Y.P. (ed.). Soil and Plant Analysis. Handbook of reference methods for plant analysis. CRC Press, Boca Raton, FL.
Isaac, R.A. & Johnson, W.C. 1976 Determination of total nitrogen in plant tissue using a block digestor J. Assoc. Off. Anal. Chem. 59 98 100
Johnson, R.S. & Crisosto, C.H. 2003 The effect of nutrient deficiencies on stone fruit production and quality. Report to the California Tree Fruit Agreement Commission. University of California, Davis, CA.
Johnson, R.S. & Uriu, K. 1989 Mineral nutrition, p. 68–81. In: LaRue, J.H. (ed.). Peaches, plums, and nectarines: Growing and handling for fresh market. University of California Cooperative Extension. Division of Agriculture and Natural Resources, Oakland, CA.
Kotze, W.A.G., Gurgen, K.H., Vanrooyen, P.C. & Dodd, M.C. 1987 Statistical evaluation of the effect of mineral-nutrition on the incidence of internal breakdown in plums J. Plant Nutr. 10 1547 1554
Meyer, G.A. & Keliher, P.N. 1992 An overview of analysis by inductively coupled plasma-atomic emission spectrometry, p. 473–516. In: Montaser, A. and D.W. Golightly (eds.). Inductively coupled plasmas in analytical atomic spectrometry. VCH Publishers, New York, NY.
Mitchell, F.G. 1987 Influence of cooling and temperature maintenance on the quality of California grown stone fruit Intl. J. Refrig. 10 77 81
Ogundiwin, E.A., Crisosto, C.H., Peace, C.P., Nicolet, C.M., Rashbrook, V.K., Gradziel, T.M., Bliss, F.A. & Parfitt, D. 2008 Leucoanthocyanidin dioxygenase gene (PpLDOX): A potential functional marker for cold storage browning in peach Tree Genet. Genomes 4 543 554
Robards, K., Prenzler, P.D., Tucker, G., Swatsitang, P. & Glover, W. 1999 Phenolic compounds and their role in oxidative processes in fruits Food Chem. 66 401 436
Singleton, V.L. & Rossi, J.A. 1965 Colorimetry of total phenolics [in grapes and wine] with phosphomolybdic-phosphotungstic acid reagents American Journal of Enology and Viticulture 16 144 158
Tjellstrom, H., Andersson, M.X., Larsson, K.E. & Sandelius, A.S. 2008 Membrane phospholipids as a phosphate reserve: The dynamic nature of phospholipid-to-digalactosyl diacylglycerol exchange in higher plants Plant Cell Environ. 31 1388 1398
U.SDA 2009 Composition of nectarines, raw. USDA National Nutrient Database for Standard Reference. 21 June 2011. <http://www.nal.usda.gov/fnic/foodcomp/cgi-bin/list_nut_edit.pl>.