Onion Inbred Line ‘B8667 A&B’ and Synthetic Populations ‘Sapporo-Ki-1 A&B’ and ‘Onion Haploid-1’

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  • 1 USDA/ARS, Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706
  • | 2 University of Ljubljana, Biotechnical Faculty, Agronomy Department, Jamnikarjeva 101, 1000 Ljubljana, Slovenia

The U.S. Department of Agriculture, Agricultural Research Service, announces the release of onion inbred ‘B8667 A&B’ and synthetic population ‘Sapporo-Ki (SKI) -1 A&B’. Both of these releases represent a cytoplasmic male-sterile (A) line with its maintainer (B) for seed propagation of male-sterile plants. ‘B8667 A&B’ is intended for production of red, long-day, well-storing hybrids. ‘SKI-1’ combines the earliness of the Japanese population ‘Sapporo-Ki’ with maintenance of cytoplasmic male sterility. The long-day synthetic population ‘Onion Haploid-1’ (‘OH-1’) is a joint release of the U.S. Department of Agriculture, Agricultural Research Service, and the University of Ljubljana, Slovenia. ‘OH-1’ is intended to serve as a responsive control for extraction of gynogenic haploids of onion.

Abstract

The U.S. Department of Agriculture, Agricultural Research Service, announces the release of onion inbred ‘B8667 A&B’ and synthetic population ‘Sapporo-Ki (SKI) -1 A&B’. Both of these releases represent a cytoplasmic male-sterile (A) line with its maintainer (B) for seed propagation of male-sterile plants. ‘B8667 A&B’ is intended for production of red, long-day, well-storing hybrids. ‘SKI-1’ combines the earliness of the Japanese population ‘Sapporo-Ki’ with maintenance of cytoplasmic male sterility. The long-day synthetic population ‘Onion Haploid-1’ (‘OH-1’) is a joint release of the U.S. Department of Agriculture, Agricultural Research Service, and the University of Ljubljana, Slovenia. ‘OH-1’ is intended to serve as a responsive control for extraction of gynogenic haploids of onion.

The U.S. Department of Agriculture, Agricultural Research Service, announces the release of onion inbred ‘B8667 A&B’ and synthetic population ‘Sapporo-Ki (SKI) -1 A&B’. Both of these releases represent a cytoplasmic male-sterile (A) line with its maintainer (B) for seed propagation of male-sterile plants. The synthetic population ‘Onion Haploid-1’ (‘OH-1’) is a joint release of the U.S. Department of Agriculture, Agricultural Research Service, and the University of Ljubljana, Slovenia.

Origin and Description

‘B8667 A&B’ is intended for production of red, long-day, well-storing hybrids. This inbred line is round in shape, dark red with color extending through the internal rings of the bulb, with good scale retention and excellent storage quality when produced on high-organic (muck) soils (Fig. 1A). ‘B8667B’ is a F1MSMS2M3 from USDA PI 262985 (‘Noord Holland Bloodred’) crossed with ‘B5361B’ (a red inbred developed by the late Dr. Clint Peterson, USDA-ARS, but never released). This inbred has a soluble solids content of 13.4 ± 0.7% and is relatively pungent at 10.7 ± 1.0 μm pyruvate per milliliter. The cytoplasmic male-sterile A line is a BC7. Testcrosses of ‘B8667B’ to a series of male-sterile hybrid female lines (MSU611–1A × MSU611B, MSU5718A × MSU8155B, and B1731A × MSU5785B) produced only red bulbs and were not significantly different for bulb yield as compared with commercial hybrids evaluated in replicated trials over 2 years at Palmyra, WI (Table 1).

Table 1.

Mean yield of onion bulbs over 2 years for commercial hybrids and testcrosses using inbred B8667B as the male.

Table 1.
Fig. 1.
Fig. 1.

Bulbs of ‘B8667 B’ (A), ‘SKI-1 B’ (B), and ‘OH-1’ (C).

Citation: HortScience horts 42, 7; 10.21273/HORTSCI.42.7.1731

‘Sapporo-Ki’ is an open-pollinated population grown on the Japanese island of Hoikkaido and has relatively high frequencies of both S cytoplasm and the dominant allele at the male-fertility restoration locus (Ms) (Havey, 1995). This synthetic population combines the early maturity of ‘Sapporo-Ki’ with maintenance of cytoplasmic male sterility. Random plants from ‘Sapporo-Ki’ were self-pollinated and testcrossed to male-sterile plants of MSU611–1A × MSU611B or MSU5718A × MSU8155B. The S1 families were evaluated for their cytoplasm using the molecular markers as described by Havey (1993a, 1995). Testcross families from N-cytoplasmic plants were scored for male-fertility restoration (Gokce and Havey, 2002). S1 families that were N-cytoplasmic and homozygous recessive at Ms were selected. Five S1 bulbs from each of eight families were caged, allowed to flower, and intercrossed using flies (Musca domestica L. from Rincon-Vitova Insectaries, Ventura, CA) as described by Havey (1993b) followed by three generations of seed increases. The cytoplasmic male-sterile A line is a BC5. Bulbs of this synthetic population are yellow (Fig. 1B) and mature ≈2 weeks earlier than commercial hybrids in Wisconsin.

‘Onion Haploid (OH) -1’ is a long-day synthetic population intended to serve as a responsive control for extraction of gynogenic haploids of onion. Random plants from the relatively responsive inbreds ‘B2923B’ and ‘B0223B’ were evaluated for gynogenic haploid production as described by Bohanec and Jakše (1999) and Jakše et al. (2003). Plants that produced relatively high numbers of gynogenic haploids were self-pollinated (Bohanec et al., 2003). Five bulbs from each of 10 S1 families (nine from ‘B2923B’ and one from ‘B0223B’) were caged, allowed to flower, and intercrossed using flies. Plants in this synthetic produced on average 12 gynogenic haploids for every 100 flowers plated. Bulbs of this synthetic population are yellow with good storage quality (Fig. 1C). All plants of ‘OH-1’ should be N-cytoplasmic and homozygous recessive at the Ms locus, although this has not been confirmed.

Availability

Breeders’ seed of ‘B8667 A&B’, ‘OH-1’, and ‘SKI-1 A&B’ from cage isolations are available Dr. M. J. Havey, USDA-ARS, Department of Horticulture, 1575 Linden Dr., University of Wisconsin, Madison WI 53706. Seed of the maintainer (B) lines have been deposited into the USDA PI System as numbers 645565 (‘8667B’), 645566 (‘OH-1’), and 645567 (‘SKI-1B’).

Literature Cited

  • Bohanec, B. & Jakše, M. 1999 Variations in gynogenic response among long-day onion (Allium cepa L.) accessions Plant Cell Rep. 18 737 742

  • Bohanec, B., Jakše, M. & Havey, M.J. 2003 Genetic analyses of gynogenetic haploid production in onion J. Amer. Soc. Hort. Sci. 128 571 574

  • Gokce, A.F. & Havey, M.J. 2002 Linkage equilibrium among tightly linked RFLPs and the Ms locus in open-pollinated onion populations J. Amer. Soc. Hort. Sci. 127 944 946

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  • Havey, M.J. 1993a A putative donor of S-cytoplasm and its distribution among open-pollinated populations of onion Theor. Appl. Genet. 86 128 134

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  • Havey, M.J. 1993b Estimation of outcrossing using house flies for fertile-by-fertile crosses in onion Allium Improvement Newsletter 3 12 14

  • Havey, M.J. 1995 Cytoplasmic determinations using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion Theor. Appl. Genet. 90 263 268

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  • Jakše, M., Havey, M.J. & Bohanec, B. 2003 Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos Plant Cell Rep. 21 905 910

Contributor Notes

This work was supported by the Agricultural Research Service of the USDA and U.S.–Slovene research project 95/10-02-ARS-13.

Names are necessary to report factually on available data; however, the U.S. Department of Agriculture (USDA) neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

To whom reprint requests should be addressed; e-mail mjhavey@wisc.edu

  • View in gallery

    Bulbs of ‘B8667 B’ (A), ‘SKI-1 B’ (B), and ‘OH-1’ (C).

  • Bohanec, B. & Jakše, M. 1999 Variations in gynogenic response among long-day onion (Allium cepa L.) accessions Plant Cell Rep. 18 737 742

  • Bohanec, B., Jakše, M. & Havey, M.J. 2003 Genetic analyses of gynogenetic haploid production in onion J. Amer. Soc. Hort. Sci. 128 571 574

  • Gokce, A.F. & Havey, M.J. 2002 Linkage equilibrium among tightly linked RFLPs and the Ms locus in open-pollinated onion populations J. Amer. Soc. Hort. Sci. 127 944 946

    • Search Google Scholar
    • Export Citation
  • Havey, M.J. 1993a A putative donor of S-cytoplasm and its distribution among open-pollinated populations of onion Theor. Appl. Genet. 86 128 134

    • Search Google Scholar
    • Export Citation
  • Havey, M.J. 1993b Estimation of outcrossing using house flies for fertile-by-fertile crosses in onion Allium Improvement Newsletter 3 12 14

  • Havey, M.J. 1995 Cytoplasmic determinations using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion Theor. Appl. Genet. 90 263 268

    • Search Google Scholar
    • Export Citation
  • Jakše, M., Havey, M.J. & Bohanec, B. 2003 Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos Plant Cell Rep. 21 905 910

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