Nitric Oxide and Nitrite Treatments Reduce Ethylene Evolution from Apple Fruit Disks

in HortScience
View More View Less
  • 1 U.S. Department of Agriculture, Agricultural Research Service, Tree Fruit Research Laboratory, 1104 N. Western Avenue, Wenatchee, WA 98801-1230

`Golden Delicious' apple [Malus sylvestris var. domestica (Borkh.)] cortex disks suspended in solutions containing a nitric oxide (•NO) donor [S-nitrosoglutathione (GSNO) or sodium nitroprusside (SNP)], •NO gas, or nitrite (KNO2) were used to identify impacts of •NO on ethylene production and NO2 on •NO and ethylene production. Treatment with GSNO or SNP reduced ethylene biosynthesis compared with control treatments containing equimolar concentrations of oxidized glutathione (GSSG) or Na4(CN)6 respectively. Apple disk exposure to •NO gas did not impact ethylene production. Treatment with NO2 resulted in increased •NO production and decreased ethylene biosynthesis. Generation of •NO increased linearly whereas ethylene generation decreased exponentially with increasing NO2 treatment concentration. •NO was enhanced in autoclaved tissue disks treated with NO2, suggesting that its production is produced at least in part by nonenzymatic means. Although this evidence shows •NO is readily generated in apple fruit disks by NO2 treatment, and ethylene synthesis is reduced by •NO/NO2 generated in solution, the exact nature of •NO generation from NO2 and ethylene synthesis modulation in apple fruit disks remains to be elucidated.

If the inline PDF is not rendering correctly, you can download the PDF file here.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 226 51 2
PDF Downloads 139 52 6