RNA Extraction Method from Fruit Tissue High in Water and Sugar

in HortScience
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  • 1 U.S. Department of Agriculture, Agricultural Research Service, South Central Agricultural Research Laboratory, P.O. Box 159, Hwy. 3 West, Lane, OK 74555
  • | 2 U.S. Department of Agriculture, Agricultural Research Service, U. S. Vegetable Laboratory, 2700 Savanna Highway, Charleston, SC 29414
  • | 3 Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843
  • | 4 University of Illinois at Urbana, Champaign Biotechnology Center, W. M. Keck Center for Comparative and Functional Genomics, 1201 W. Gregory Dr., Room 356, Urbana, IL 61801

RNA isolation from ripe fruit can be complicated by high concentrations of sugar and water. These sugars interfere with RNA extraction often resulting in low RNA quality and quantities, and high water concentrations dilute the RNA, making isolation difficult. We report a simple but novel method by which the majority of the excess sugar and water in mature fruit of tomato (Lycopersicon esculentum Mill.), watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai], and muskmelon (Cucumis melo L.) can be easily removed from tissue before RNA extraction. This method produced quality RNA in a shorter time than the currently accepted method for fruit tissue RNA isolation and does not require liquid nitrogen or a freeze dryer.

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