TESTING ANTIFUNGAL COMPETENCY OF COMPOUNDS AGAINST SOME POSTHARVEST PATHOGENS USING THE DISC ASSAY METHOD

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  • 1 USDA–ARS Citrus and Subtropical Products Laboratory, Winter Haven, FL 33884

There are many methods to test the efficiency of antimicrobial compounds. Our goals were to perform screens of several natural, experimental compounds and evaluate their effects on postharvest pathogenic fungi isolated from fruit. This screen was the first test in series that would allow us to see if these experimental compounds had potential use as components in a coating or as a preharvest treatment to help insure postharvest fruit quality. The disc assay method was chosen as a preliminary method for our screen as most of our compounds are water soluble and this method is straightforward, efficient and easy to interpret. This poster describes the testing of natural compounds against problematic postharvest fungi using the disc assay as a screening method. The results of various compounds are shown via the formation of a prominent zone of inhibition. Comparisons are also shown of non-responsive compounds to Penicillium digitatum and Geotrichum citri-aurantii. The clarity of using this method for step-wise dilutions of the anti-fungal compounds is shown.

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