To induce multiple shoots from habanero pepper (Capsicum chinense Jacq.), nodes and stem segments were cultivated in MS medium supplemented with varying concentrations of kinetin, benzyladenine, and thidiazuron. The effect of the age of the explant in the medium on shoot formation and their latter development into plants was assessed. Ethylene concentration was measured along the experiments. Thidiazuron was the key growth regulator in the process, which at 3.4 μm induced seven to eight shoots that developed into healthy plants per explant. Plantlets in nonventilated vessels, where ethylene concentration was 0.25 ± 0.1102 μL·L–1, showed early defoliation and the formation of calli on the leaves and stems.