Fruits of `Tsugaru' (an early maturing cultivar), `Hongro' (mid-season cultivar), and `Fuji' (late cultivar), were harvested at different times of the year, depending upon their maturity, and treated with 1-MCP at 0.0, 0.5, 1.0, and 2.0 ppm for 8-24 hours. Fruits were also treated with 1-MCP at different times after harvest. Portions of 1-MCP-treated apples were also treated with ethylene in order to study the interaction between 1-MCP and ethylene. In other experiments, fruits were treated with ethylene first and then treated again with 1-MCP. Major results are as follows. Treatment of 1-MCP greatly retarded the senescence of `Tsugaru' apple stored at room temperatures as compared with the control. The sooner the time of 1-MCP treatment after harvest of fruit, the greater was the 1-MCP effect. In contrast to the time of 1-MCP treatment, the concentration of 1-MCP and duration of 1-MCP infiltration in a closed chamber exhibited only a minor effect. Ethylene treatment immediately before and/or after the 1-MCP treatment showed only the 1-MCP effect, thus clearly showing that 1-MCP treatment could completely reverse or counter the ethylene effect in `Fuji' apples. Repeated treatments of 1-MCP after a certain period of low temperature storage of `Fuji' apples were more effective than a single treatment. Parameters related with fruit quality will be discussed in detail.
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