A cold acclimatization mechanism regulated by the accumulation of mRNAs and proteins has been tentatively identified in japanese spurge (Pachysandra terminalis Sieb. & Zucc.). Two polypeptides and several cDNA fragments were observed in leaf tissue after acclimation. When these proteins were probed with type III fish antifreeze antibodies, an immune-cross reaction occurred. Nonacclimatized young leaves and stems of japanese spurge survived 20-minute exposures at -5 °C. Although newly emerged leaves and stems were damaged, plants resumed growth at higher temperatures. After acclimation by gradual cold treatments (4 to -5 °C), new proteins began to accumulate in young leaves and plants were more tolerant to extended treatments at -5 °C. Changes in accumulation of proteins and mRNA in leaf tissue of japanese spurge appear to be an adaptation mechanism to subfreezing conditions. This is the first report of the immune-cross reaction between antibodies of type III fish antifreeze proteins and plant proteins
If the inline PDF is not rendering correctly, you can download the PDF file here.
To whom all correspondence should be directed; e-mail email@example.com.