Sequences of Probes Revealing Mapped Restriction Fragment-length Polymorphisms in Cucumber

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  • 1 Agricultural Research Service, U.S. Department of Agriculture, Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706

PstI-genomic and cDNA clones revealing mapped restriction fragment-length polymorphisms (RFLP) in cucumber (Cucumis sativus L.) were sequenced in order to ensure that these clones remain available and to determine if any clones showing genetic linkage in cucumber are physically linked in Arabidopsis thaliana. Sequence comparisons using translated searches revealed that 80% of the cucumber cDNA clones showed significant (≤e-20) similarities to Arabidopsis expressed sequence tags (ESTs) or genomic sequences, as opposed to relatively few (32%) of the cucumber genomic clones. Two clones revealing RFLPs linked at 2 cM in cucumber showed significant (≤e-20) similarities to sequences separated by 347,616 basepairs on chromosome 4 of Arabidopsis.

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Contributor Notes

USDA research geneticist and professor of horticulture. To whom correspondence should be addressed; e-mail mjhavey@wisc.edu.
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