Molecular Characterization of Moroccan Fig Germplasm Using Intersimple Sequence Repeat and Simple Sequence Repeat Markers To Establish A Reference Collection

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  • 1 Conservatoire Botanique National Méditerranéen de Porquerolles (CBNMP), Parc National de Port-Cros, Castel Ste Claire, F 83418 Hyères cedex, France
  • 2 INRA, Programme Arboriculture Fruitière, BP 578, Meknès Morocco
  • 3 Ecologie et Biologie des Populations, Faculté des Sciences de Tétouan, B.P 2062, M'hannech II Tétouan, Morocco
  • 4 INRA, Programme Arboriculture Fruitière, BP 578, Meknès Morocco
  • 5 Conservatoire Botanique National Méditerranéen de Porquerolles (CBNMP), Parc National de Port-Cros, Castel Ste Claire, F 83418 Hyères cedex, France
  • 6 Centre d'Ecologie Fonctionnelle et Evolutive, CNRS, 1919 route de Mende 34293 Montpellier Cedex 5, France

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.

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Contributor Notes

To whom correspondence should be addressed. Current address: UMR 1098 Biologie du Développement des Espèces Pérennes Cultivées (BEPC), Bat. 33, INRA, 2, place Viala, 34 060 Montpellier cedex 1, France; e-mail khadari@ensam.inra.fr.
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