Research was conducted in two studies at the Clemson University Greenhouse Complex, Clemson, S.C., with the objective of evaluating `TifEagle' bermudagrass (Cynodon dactylon × C. transvaalensis) response to paclobutrazol. TifEagle bermudagrass plugs were placed in 40 cm polyvinylchloride containers, with 20.3-cm-diameters and built to U.S. Golf Association specifications with 85 sand: 15 peatmoss (by volume) rootzone mix. Paclobutrazol was applied to separate containers at 0, 0.14, 0.28, and 0.42 kg·ha-1 (a.i.) per 6 weeks. Minor phytotoxicity occurred with 0.14 kg·ha-1 applications, but turf quality was unaffected. Severe bermudagrass phytotoxicity occurred from paclobutrazol at 0.28 and 0.42 kg·ha-1. Total clipping yield from 12 sampling dates was reduced 65%, 84%, and 92% from 0.14, 0.28, and 0.42 kg·ha-1, respectively. Root mass after 12 weeks was reduced 28%, 45%, and 61% for turf treated 0.14, 0.28, and 0.42 kg·ha-1, respectively. Paclobutrazol reduced root length 13%, 19%, and 19% by 0.14, 0.28, and 0.42 kg·ha-1, respectively. Turf discoloration and negative rooting responses advocate caution when using paclobutrazol on `TifEagle' bermudagrass. Chemical names used: (+/-)-(R*,R*)-ß-[(4-chlorophenyl) methyl]-alpha-(1, 1-dimethyl)-1H-1,2,4,-triazole-1-ethanol (paclobutrazol).