Aluminum toxicity is a major limiting factor for turfgrass establishment and growth when soil pH is <5.0. Limited information on aluminum resistance is available among warm-season turfgrasses and these turfgrasses often grow in the areas with acid soil conditions. The objectives of this study were 1) to evaluate seeded bermudagrass (Cynodon dactylon L.) cultivars for the ability to tolerate a high level of aluminum and 2) to measure the extent of aluminum damage to the root systems. In total, 16 bermudagrass cultivars were evaluated under greenhouse conditions using a solution culture and an acid Tatum soil (Clayey, mixed, thermic, typic, Hapludult). The soil had pH 4.4% and 69% exchangeable aluminum. A concentration of 640 μm aluminum and a pH 4.0 was used for solution culture. The grasses were grown for 28 days in solution culture; 28 days in the acid Tatum soil; and 78 days in the acid Tatum soil before harvesting. Aluminum resistance was determined by measuring the longest root length, the longest shoot length, dry root weight, dry shoot weight, and shoot to root ratio in comparing the control to obtain the relative Al resistance among the cultivars. The results indicate that seeded bermudagrass cultivars differ in their aluminum resistance.