Seed Priming Effect on Symbiotic Germination and Seedling Development of Orchis palustris Jacq.

in HortScience
Authors:
Ahmet EsitkenDepartment of Horticulture, Ataturk University, Faculty of Agriculture, 25240 Erzurum, Turkey

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Sezai ErcisliDepartment of Horticulture, Ataturk University, Faculty of Agriculture, 25240 Erzurum, Turkey

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Cafer EkenDepartment of Plant Protection, Ataturk University, Faculty of Agriculture, 25240 Erzurum, Turkey

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David TayOrnamental Plant Germplasm Center, The Ohio State University, 670 Vernon Tharp Street, Columbus, OH 43210

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Seeds of Orchis palustris Jacq. were primed for 1- to 5-day in polyethylene glycol (PEG-6000) solutions at -0.5, -1.0 or -1.5 MPa. The seeds were symbiotically germinated with BNR 8-3 mycorrhizal fungus on oatmeal agar at 22 °C. In general, priming hastened rapid germination. At -1.5 MPa water potential, the first to germinate was eight days compared to 18 days for the control. Percentage germination increased as priming water potential decreased, and the percentage germination was 55%, 58%, and 65%, at -0.5, -1.0, and -1.5 MPa, respectively, versus 43% for the nonprimed control. Priming duration from 1 to 5 days had little effect on germination performance. The best germination percentage (68%) was obtained from 1 day at -1.5 MPa treatment.

Contributor Notes

Corresponding author, e-mail aesitken@atauni.edu.tr.
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