Potted grapevines (Vitis vinifera L. `Chardonnay') were inoculated with conidial suspensions of the grapevine pathogen causing powdery mildew of grape (GPM) (Uncinula necator (Schw.) Burr.). Leaves of inoculated and noninoculated vines were studied for the effects of varying light (PAR) and CO2 concentrations on factors affecting carbon assimilation. GPM reduced carboxylation efficiency (k), net CO2 assimilation rate (A), stomatal conductance (gs), and internal CO2 concentration (Ci) under ambient CO2, Amax at >900 ppm CO2, stomatal limitations to A (lg), and photochemical efficiency (Φ) on diseased leaves, while having no effect on the CO2 compensation point (Γ) or the light compensation point (cp). GPM had no significant effect on chlorophyll fluorescence (Fv/Fm).