Segregation of Scab Resistance in Three Apple Populations: Molecular Marker and Phenotypic Analyses

in HortScience
View More View Less
  • 1 Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907

The codominant PCR marker AL07-SCAR closely linked to the Vf gene for scab resistance was used to genotype seedlings in three apple populations in which each parent (`GoldRush', `Enterprise', `Pristine', and CQR10T17) was resistant to apple scab. The marker was used to predict the genotype at the Vf locus. Each parent was heterozygous. In two populations (CQR10T17 × `GoldRush' and `Pristine' × `GoldRush') seedlings segregated 1:2:1 for fragments associated with VfVf:Vfvf:vfvf as predicted by Mendelian segregation. However, in another population (`GoldRush' × `Enterprise') the ratio was 1.5:1:1.5, suggesting some type of selection against heterozygotes. Fruiting seedlings were rated for the presence of fruit scab. No scab was observed on seedlings homozygous for the PCR marker linked to Vf, a small amount of scab was observed on one heterozygous seedling out of 35, and 22 of 26 seedlings that were homozygous recessive, had fruit scab.

If the inline PDF is not rendering correctly, you can download the PDF file here.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 64 22 1
PDF Downloads 86 43 2