A New Disease in Limonium hybrids. I. Molecular Identification

in HortScience
View More View Less
  • 1 Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 52050, Israel
  • | 2 The Plant Protection and Inspection Services, Ministry of Agriculture, Bet Dagan 50250, Israel
  • | 3 Northern Arava Research and Development, Sapir, D.N. Arava 86825, Israel
  • | 4 Department of Entomology, ARO, Gilat Research Center, D.N. Negev 85280, Israel

Yellows diseases in ornamental crops have become more common in Israel, and phytoplasmas have been detected in several crops. Recently, symptoms typical of a phytoplasma infection were observed on a large number of Limonium hybrids grown in commercial fields in Israel. Examination of samples from diseased plants by electron microscopy revealed the presence of pleiomorphic membrane-bound bodies in the phloem cells. Diseased plants were additionally analyzed by PCR using universal and nested primers and revealed upon sequencing products of 1143, 788 and 722 bp (Li-IL, Li-b2-IL, Li-v3-IL, respectively). Analysis of the PCR products, associated with infected Limonium, clustered within two major groups of phytoplasmas (16SrV and 16SrIX), elm yellows and almond witches broom. This is the first published record of these phytoplasmas in Israel.

Contributor Notes

Corresponding author; e-mail abedgera@volcani.agri.gov.il.
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 91 17 1
PDF Downloads 165 46 0