Enhanced Histological Technique for Observation of Spathe Pigmentation in Anthurium species and Hybrids

in HortScience
Authors:
Jennifer A. Ehrenberger*The Ohio State Univ., Ornamental Plant Germplasm Center, Columbus, OH 43210-1086

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Adelheid R. KuehnleUniv. of Hawaii at Manoa, Tropical Plant and Soil Sciences, Honolulu, HI 96822-2279

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A hybridization strategy for certain coloration could be developed based on accurate histological information of parental material together with the knowledge of heritability of color and color intensity. A sample of 12 Anthurium species and hybrids were histologically examined for pigmentation in spathes using a new method employing vacuum infiltration of spathe tissue with polyethylene glycol (PEG) prior to cross-sectioning. PEG infiltration displaces intercellular air spaces between cells. This method greatly improved the clarity of the cross sections and consequently improved observations of spatial localization of anthocyanins and chloroplasts. This infiltration method accurately identified the spatial localization of pigments for future breeding reference, notably among Anthurium species.

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