Morphogenetic Response of Lilium michiganense to Four Auxins In Vitro

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  • 1 Chicago Botanic Garden, Ornamental Plant Research, Glencoe, IL 60022
  • 2 Chicago Botanic Garden, Ornamental Plant Research, Glencoe, IL 60022

Shoot, root, and callus induction were examined in the North American lily, Lilium michiganense, in response to treatment with four auxins. Seed from controlled crosses were aseptically excised from slightly immature capsules and cultured in vitro on Murashige and Skoog basal medium and vitamins with 30 g/l sucrose, 7.0 g/l agar, and a pH = 5.7. Seed were maintained at 20 °C with a 14-h photoperiod. After 5.0-5.5 months, leaves and roots were removed from seedlings, the bulbs transversely sectioned, then the bulb sections cultured cut-surface down on the identical medium supplemented with 0.0, 1.0, 2.0, 4.0, or 8.0 μm dicamba, picloram, K-NAA, or 2,4-D. PGRs were added to medium prior to autoclaving except dicamba which was dissolved in 50% ethanol and added after medium autoclaving. 16 explants were utilized for each treatment. The experiment was conducted three times. Morphogenetic response (# of shoots produced, % of explants forming roots, and % of explants forming callus) was tabulated 4 months after treatment. Shoot formation was promoted by treatment with dicamba, picloram, and K-NAA in comparison to the control (2.5 shoots/explant). Shoot formation varied significantly in response to individual dicamba, picloram, and 2,4-D concentrations. A maximum of 7.9 shoots per explant was promoted by 4.0 μm K-NAA and 1.0 μm dicamba, respectively. Root and callus formation also varied significantly between auxin treatments. Root formation was inhibited by dicamba, picloram, and 2,4-D treatments in comparison with the control (100% rooting); callus formation was promoted by dicamba, picloram, and K-NAA treatments in comparison with the control (15% callusing).

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