Plant Regeneration from Leaf of Streptocarpus × hybridus Voss. Cultured In Vitro

in HortScience
View More View Less
  • 1 Univ. of Agricultural Sciences and Medicine Veterinary Cluj-Napoca, Horticulture/Flori-culture, Cluj-Napoca, Cluj, 400372, Romania
  • | 2 Univ. of Agricultural Sciences and Medicine Veterinary Cluj-Napoca, Horticulture/Biotechnology, Cluj-Napoca, Cluj, 400372, Romania
  • | 3 Univ. of Agricultural Sciences and Medicine Veterinary Cluj-Napoca, Horticulture/Informatics, Cluj-Napoca, Cluj, 400372, Romania

The Streptocarpus is propagated ease vegetatively from leaf cuttings all year round, but is grown on a very limited scale commercially in Romania. Successful protocol for direct shoot regeneration from in vitro Cape primrose (Streptocarpus × hybridus Voss.) leaf explants has been developed. The ease of tissue culture propagation can promote Streptocarpus production and facilitated the rapid introduction of this new species. Adventitious shoot regeneration was inducted in vitro on MS basal medium, using different concentration of NAA (1, 1.5, 2 mg·L-1) and cyokinin TDZ (0.1, 0.5, 1 mg·L-1). High frequency regeneration was obtained from leaves when cultured in the media supplemented with 1 mg·L-1 NAA plus 0.5 mg·L-1 TDZ and the percent of regeneration resulted is between 70% to 100%. Complete plantlets were acclimatized and successfully transplanted to glasshouse conditions. The total duration of the cycle from leaf explants through complete plantlets was 10 weeks.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 64 17 1
PDF Downloads 97 36 0