Poor germination in Rosa has been an obstacle to breeding programs for years. Rose breeders generally stratify rose seed under cool, moist conditions for 4-10 weeks by planting directly into the seedling flat/bed or in a small container followed by planting the germinating seed into the seedling flat/bed. This experiment used 9 genotypes and compared these two approaches combined with variations in the stratification media (sand, perlite, sphagnum moss and Sunshine Mix #4). Over all stratification media and genotypes, germination was not influenced by whether the seed was stratified directly in the seedling flat/bed or in a small container. However, the process of transplantation of the delicate germinating seed from the small container to the flat/bed resulted in greater mortality of the germinating seedlings. he stratification media affected the germination of the rose seed. Sunshine Mix #4 gave the best germination as compared to all other media types tested. As expected the germination of the genotypes varied greatly, ranging from 0.7% to 37.1%.