Genetically modified herbicide-tolerant (GMHT) and non-GM chile pepper plants (Capsicum annuum L. cv. `Subicho') were grown in an isolated GMO field to evaluate horticultural characteristics. Phosphinotricin acetyltransferase (bar gene), which has a function of herbicide-resistance in plant, was introduced into chile pepper plants using the protocol of Agrobacterium-mediated transformation. Thirty nine characteristics were evaluated, consisting of 14 qualitative, 18 quantitative and 7 other characters. The evaluations were achieved by visual assessment for qualitative characteristics and numerical measurement for quantitative ones. The GMHT and non-GM plants did not differ in the 39 characteristics tested. Pollen viability and germination rate were not significant different between the GMHT plants and the non-GM plants. These results indicated that genetic transformation of bar gene into the chile pepper did not affect those horticultural characteristics and pollen viability.