The influence of two irrigation treatments during nursery production on the post-transplant development of Lotus creticus subsp. cytisoides was studied. The treatments lasted 96 days and consisted of irrigating 2 days/week with a total of 2.3 L of water per plant over the whole nursery period (T-2) or irrigating six days per week with a total of 7 L of water per plant (T-6). T-2 plants had greater root length: shoot length ratio and higher percentage of brown roots, an indicator of more resistance to post-transplant stress. Minirhizotrons revealed more active root growth in the surface soil of the T-2 plants, although the plants of both treatments rapidly colonized the whole soil depth studied (0-160 cm deep). T-2 plants had greater stem length growth per unit of soil area covered.