Three-year-old field-grown 'Concord' (Vitis labruscana Bailey) grapevines were destructively harvested at eight growth stages during 1998 to quantify growth, carbohydrate distribution, and nutrient concentrations of different organs. The roots were the major storage organ for carbohydrates and nutrients, accounting for 84% of the starch and 75% of nitrogen stored in the vines at the beginning of the season. About 78% of the reserve starch in the vine was used for prebloom root and shoot growth. Early-season fine-root growth was a sink for stored vine nitrogen; however, the fine roots quickly became a nitrogen uptake source, providing at least 84% of the spring growth nitrogen. Total root biomass increased from bloom to leaf fall, but reserve carbohydrates and nutrients lost in the prebloom period did not begin to recover in roots until the end of rapid shoot development in late July. Crop removal at harvest, and a late-season root flush, further increased vegetative carbohydrate and nutrient reserves in the short postharvest period.