The effects of trinexapac-ethyl (TE) on the anatomical and biochemical composition of turfgrasses and their implications for its field use are poorly understood. Two greenhouse experiments were conducted to determine if application of TE increased Kentucky bluegrass (Poa pratensis L.) leaf blade cell density, chlorophyll concentration, or structural carbohydrate content. Kentucky bluegrass (KB) sod was harvested from the field, established in greenhouse pots, and sprayed with 0.27 kg·ha-1 a.i. TE. Leaf blade samples were collected 4 weeks after treatment (WAT), fixed in glutaraldehyde, and embedded in Spurr resin. Photomicrographs of longitudinal leaf blade sections were used to determine cell density, cell length, and cell width. Chlorophyll and structural carbohydrate contents were determined at 2 and 4 WAT. Treatment with TE increased cell density and chlorophyll-b concentration, while reducing cell length, but structural carbohydrate content was unaffected. Chemical name used: 4-cyclopropyl-α-hydroxy-methylene-3,5-dioxo-cyclohexanecarboxylic acid ethyl ester (trinexapac-ethyl).