Small Inflorescence Bulbils Are Best for Micropropagation and Virus Elimination in Garlic

in HortScience
Authors:
M. EbiDepartment of Horticultural Sciences, Hokkaido University, Sapporo 060-8589, Japan

Search for other papers by M. Ebi in
ASHS
Google Scholar
PubMed
Close
,
N. KasaiDepartment of Horticultural Sciences, Hokkaido University, Sapporo 060-8589, Japan

Search for other papers by N. Kasai in
ASHS
Google Scholar
PubMed
Close
, and
K. MasudaDepartment of Horticultural Sciences, Hokkaido University, Sapporo 060-8589, Japan

Search for other papers by K. Masuda in
ASHS
Google Scholar
PubMed
Close

Garlic (Allium sativum L.) plants were cultured from immature inflorescence bulbils. The primordia of bulbils appeared initially as protuberances on reproductive apices, swelled, and then formed bulbils with protective leaves. Excised bulbils sprouted on Murashige and Skoog's medium with 5.4 μm naphthaleneacetic acid (NAA) and grew into plantlets. The frequency of sprouting in culture increased with the development of bulbils before excision, and immature bulbils 0.4 to 2.4 mm in diameter sprouted at a frequency of >85%. A dot immunoblot assay revealed a remarkable reduction in levels of garlic mite-borne mosaic virus in plantlets grown from immature bulbils, suggesting that such bulbils might be suitable for the propagation of virus-free garlic plants.

Contributor Notes

to whom reprint requests should be addressed (e-mail: kmasuda@res.agr.hokudai.ac.jp).
  • Collapse
  • Expand