556 In Vitro Anther Culture of Cucumber (Cucumis sativus L.)

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  • 1 Dept. of Horticulture, Assiut Univ., Assiut, Egypt

This experiment was performed at the Tissue Culture Laboratory of the Horticulture Dept. of the Faculty of Agriculture at Assiut Univ., Egypt. After several attempts to determine the proper stage of buds for collection of pollen, we determined that the tetrad stage was most suitable. The pollen was cultured on either MS or B5 liquid or solid media (7% agar). Both media were used as basic salts or supplemented with growth regulators. The four growth substances were BA, NAA, K, and 2,4-D. Each growth substance was added to the medium separately as follow: BA, NAA at 15, 10, or 5 ppm; K at 0.1, 1, 2, or 5 ppm; and 2,4-D at 0.5, 1, or 5 ppm. The solidified medium was superior to the liquid medium at all the treatments that were used for callus formation. Using B5 medium did not result in any callus. The highest value of callus formation was obtained when MS medium supplemented with BA at 5 ppm. Moreover, the callus that was grown on the MS medium that had BA at 5 or 10 ppm developed a merstim tip. The control treatment produced calluses but did not develop any meristem tips. This process can be used to develop haploid plants.

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