332 Enhancement of Microtuber Production in Temporary Immersion Bioreactors with Ancymidol

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  • 1 Centre de Recherche en Horticulture, Laval Univ., Envirotron, QC, G1K 7P4, Canada

Microtubers (Solanum tuberosum cv Snowden) were produced in 1-L jar fermentors using a two-step method consisting of a shoot multiplication phase (21 days) followed by a tuberization phase (25 days). The plantlets were immersed in Murashige and Skoog (MS) liquid medium for 3.5 min every 4 h. Low concentrations of ancymidol (anti-gibberellic substance), particularly during the shoot multiplication phase, were essential for tuber initiation and development. A continuous supply of 2 μMol ancymidol during the two phases of culture decreased plant height, but produced >100 microtubers per jar. Although the tuber development phase was short (25 d), 25% of the microtubers produced were >0.5 g with 17.5% to 18.0% of dry-matter content.

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