Selected physiological and anatomical characteristics of four chilling-tolerant sweetpotato genotypes were evaluated. Although the genotypes were considered highly tolerant to chilling, it was proposed that differences in their mechanism for tolerance existed. A genotype temperature interaction for chlorophyll fluorescence ratio was observed when the plants were exposed to 5 °C. Genotype differences were found for electrolyte leakage and peroxidase activity. There were no differences found for fatty acid percentage composition of the glycolipid or the phospholipid fraction from leaf samples. There were no differences in diffusive resistance and transpiration rate among the genotypes; however, stomata density, leaf shrinkage, and specific leaf weight differed among the genotypes. Differences were also found among the genotypes for percent leaf dry weight, leaf thickness, and cellular structure of the leaf. It was concluded that the basis or mechanism for chilling tolerance was not the same for the four genotypes tested; therefore, combining traits for tolerance could lead to higher tolerance levels.