191 Use of the Minolta SPAD-502 to Determine Chlorophyll Concentration in Ficus benjamina L. and Populus deltoides Marsh Leaf Tissue

in HortScience
Authors:
Felix Loh1Dept. of Floriculture, Ornamental Horticulture, Cornell Univ., Urban Horticulture Institute, Ithaca, NY 14853;2 Dept. of Environmental Horticulture, Univ. of Florida, Gainesville, FL 32611-0670; 3Dept. of Floriculture, Ornamental Horticulture, Cornell Univ., Urban Horticulture Institute, Ithaca, NY 14853

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Jason Grabosky1Dept. of Floriculture, Ornamental Horticulture, Cornell Univ., Urban Horticulture Institute, Ithaca, NY 14853;2 Dept. of Environmental Horticulture, Univ. of Florida, Gainesville, FL 32611-0670; 3Dept. of Floriculture, Ornamental Horticulture, Cornell Univ., Urban Horticulture Institute, Ithaca, NY 14853

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Nina Bassuk1Dept. of Floriculture, Ornamental Horticulture, Cornell Univ., Urban Horticulture Institute, Ithaca, NY 14853;2 Dept. of Environmental Horticulture, Univ. of Florida, Gainesville, FL 32611-0670; 3Dept. of Floriculture, Ornamental Horticulture, Cornell Univ., Urban Horticulture Institute, Ithaca, NY 14853

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The Minolta SPAD meter has been used to evaluate chlorophyll concentration in plant material to provide an inexpensive method to collect rapid, nondestructive data. Correlations of SPAD data and chlorophyll concentrations in corn have been very accurate r2 = 0.95), and can be used to monitor plant nutrient status as a function of chlorophyll concentration. There has been evidence that the calibrated accuracy of the SPAD meter is diminished at low and high concentrations of chlorophyll. Our study attempted to build the same type of background information for two tree species for use in evaluating plant response in experimental media experiments. Ficus benjamina L. and Populus deltoides Marsh were grown in containers of varied media. Leaf tissue was measured with a Minolta SPAD-502, and the tissue was then removed and processed with N,N-dimenthylformamide for analysis in a spectrophotometer. The remainder of the leaf sample was analyzed in an ICAP for tissue nutrient levels. Data were analyzed to evaluate the usefulness of the SPAD meter for woody plant leaf tissue evaluation and to develop calibration curves for use in future studies. There was a positive correlation (r2 = 0.943 in Ficus) between SPAD data and combined concentrations of chlorophyll a and b. Accuracy of the SPAD data was diminished when chlorophyll concentrations were low (SPAD <20, chorophyll <450 μg·mL-1) and high (SPAD >45, chorophyll >1350 μg·mL-1)..

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