149 Effects of Plant Growth Retardants on the Growth of Aerides japonicum Cultured in Vitro

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  • 1Dept. of Horticulture, Kyungpook National Univ., Taegu 702-701, Korea; 2Dept. of Horticulture, Joongbu Univ., Kumsan 312-940. Korea; 3Dept. of Life Resources, Catholic Univ., of Taegu-Hyosung, Kyungsan 712-702, Korea;4 Dept. of Horticulture, Taegu Univ., Kyungsan 712-714, Korea

This experiment was conducted to identify the effect of various growth retardants on the growth of Aerides japonicum in vitro. Paclobutrazol was found the most effective retardant for reducing the leaf growth of seedling. Ancymidol and uniconazole also showed retarding effects on leaf growth of one, whereas Daminozide didn't. When growth retardants were added to culture medium, leaf length of seedlings was gradually shortened and leaf width became wider than that of control. However, root length was shorter and number of roots and root diameter were greatly increased. On the contrary, at 0.05 and 0.1 ppm uniconazole, growth of leaf and root were enhanced. It was showed that the possibility of using as an additive for good growth of Aerides japonicum seedling in vitro. The activity of GA-like substances was higher in the portion in which growth of seedlings were promoted. It was identified by anatomical observations that the number of stomata and thickness of cell layer in leaf were increased by treatment of retardants.

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